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10.1002/jmv.21962

http://scihub22266oqcxt.onion/10.1002/jmv.21962
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21181930!7166515!21181930
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suck abstract from ncbi


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pmid21181930      J+Med+Virol 2011 ; 83 (2): 322-30
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  • A novel real-time PCR system for simultaneous detection of human viruses in clinical samples from patients with uncertain diagnoses #MMPMID21181930
  • Katano H; Kano M; Nakamura T; Kanno T; Asanuma H; Sata T
  • J Med Virol 2011[Feb]; 83 (2): 322-30 PMID21181930show ga
  • A novel simultaneous detection system for human viruses was developed using a real-time polymerase chain reaction (PCR) system to identify causes of infection in clinical samples from patients with uncertain diagnoses. This system, designated as the "multivirus real-time PCR," has the potential to detect 163 human viruses (47 DNA viruses and 116 RNA viruses) in a 96-well plate simultaneously. The specificity and sensitivity of each probe-primer set were confirmed with cells or tissues infected with specific viruses. The multivirus real-time PCR system showed profiles of virus infection in 20 autopsies of acquired immunodeficiency syndrome patients, and detected frequently TT virus, cytomegalovirus, human herpesvirus 6, and Epstein-Barr virus in various organs; however, RNA viruses were detected rarely except for human immunodeficiency virus-1. Pathology samples from 40 patients with uncertain diagnoses were examined, including cases of encephalitis, hepatitis, and myocarditis. Herpes simplex virus 1, human herpesvirus 6, and parechovirus 3 were identified as causes of diseases in four cases of encephalitis, while no viruses were identified in other cases as causing disease. This multivirus real-time PCR system can be useful for detecting virus in specimens from patients with uncertain diagnoses.
  • |Acquired Immunodeficiency Syndrome/diagnosis/virology[MESH]
  • |Adult[MESH]
  • |Aged[MESH]
  • |DNA Primers[MESH]
  • |DNA Viruses/genetics/*isolation & purification[MESH]
  • |DNA, Viral/analysis[MESH]
  • |Female[MESH]
  • |HIV-1/genetics/isolation & purification[MESH]
  • |Humans[MESH]
  • |Male[MESH]
  • |Middle Aged[MESH]
  • |Polymerase Chain Reaction/*methods[MESH]
  • |RNA Viruses/genetics/*isolation & purification[MESH]
  • |RNA, Viral/analysis[MESH]
  • |Sensitivity and Specificity[MESH]
  • |Virus Diseases/*diagnosis[MESH]


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