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10.1128/JVI.01708-10

http://scihub22266oqcxt.onion/10.1128/JVI.01708-10
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21159861!3067811!21159861
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suck abstract from ncbi


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pmid21159861      J+Virol 2011 ; 85 (5): 2463-8
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  • Inclusion of the central exon of parvovirus B19 precursor mRNA is determined by multiple splicing enhancers in both the exon and the downstream intron #MMPMID21159861
  • Guan W; Cheng F; Huang Q; Kleiboeker S; Qiu J
  • J Virol 2011[Mar]; 85 (5): 2463-8 PMID21159861show ga
  • Alternative splicing of the precursor mRNA (pre-mRNA) of human parvovirus B19 (B19V) plays a key role in posttranscriptional regulation of B19V gene expression. We report that the central exon of the B19V pre-mRNA is defined by three GAA motif-containing exonic splicing enhancers and a G/GU-rich intronic splicing enhancer that lies adjacent to the second donor site. Moreover, targeting of morpholino antisense oligonucleotides to the two splicing enhancers surrounding the second donor site led to a significant reduction in splicing at this donor site during B19V infection of permissive CD36(+) erythroid progenitor cells.
  • |*Alternative Splicing[MESH]
  • |*Enhancer Elements, Genetic[MESH]
  • |*Exons[MESH]
  • |*Introns[MESH]
  • |Base Sequence[MESH]
  • |Erythema Infectiosum/virology[MESH]
  • |Erythroid Precursor Cells/virology[MESH]
  • |Humans[MESH]
  • |Molecular Sequence Data[MESH]
  • |Parvovirus B19, Human/*genetics[MESH]
  • |RNA Precursors/*genetics[MESH]
  • |RNA, Messenger/genetics[MESH]


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