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suck abstract from ncbi


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pmid21077443      Pol+J+Vet+Sci 2010 ; 13 (1): 143-9
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  • Survival of Corynebacterium pseudotuberculosis within macrophages and induction of phagocytes death #MMPMID21077443
  • Stefanska I; Gierynska M; Rzewuska M; Binek M
  • Pol J Vet Sci 2010[]; 13 (1): 143-9 PMID21077443show ga
  • Since C. pseudotuberculosis is a facultative intracellular pathogen the aim of this study was focused on evaluating mechanisms that allowed these bacteria to survive in macrophages and determining their influence on induction of cell death. The influence of Corynebacteria on the programmed cell death of macrophages was determined on the basis of induction the autophagy and apoptosis in the cultures of murine macrophage cell lines J774 infected with bacteria. Corynebacterium pseudotuberculosis strains could survive within macrophages more than 48 hours. During that time bacteria were released as a result of the process that lead to death of phagocytes. This property varied among studied strains. There was no increase of microtubule-associated protein I light chain 3 (MAP I LC3) activity in macrophages infected with examined strains comparing with uninfected cultures and cultures treated with autophagy inducer (rapamycin) that served as negative and positive controls, respectively. The study with confocal microscopy did not show the increasing of caspase-3 activity in the infected macrophages and their nucleus did not reveal the fragmentation.
  • |Animals[MESH]
  • |Cell Death/*physiology[MESH]
  • |Cell Line[MESH]
  • |Corynebacterium pseudotuberculosis/*physiology[MESH]
  • |Macrophages/*microbiology[MESH]
  • |Mice[MESH]


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