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10.1186/1743-422X-7-272 http://scihub22266oqcxt.onion/10.1186/1743-422X-7-272 20950445!2965155!20950445     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Virol+J 2010 ; 7 (ä): 272 Nephropedia Template TP {{tp|p=20950445|t=2010. Human parvovirus PARV4 DNA in tissues from adult individuals: a comparison with human parvovirus B19 (B19V) |pdf=|usr=}}{{20950445}} gab.com Text Human parvovirus PARV4 DNA in tissues from adult individuals: a comparison with human parvovirus B19 (B19V) JO: Virol J http://www.kidney.de/pget.php?&tw=1&pmid=20950445 #FOAvip BACKGROUND: PARV4 is a new member of the Parvoviridae family not closely related to any of the known human parvoviruses. Viremia seems to be a hallmark of PARV4 infection and viral DNA persistence has been demonstrated in a few tissues. Till now, PARV4 has not been associated with any disease and its prevalence in human population has not been clearly established. This study was aimed to assess the tissue distribution and the ability to persist of PARV4 in comparison to parvovirus B19 (B19V). RESULTS: PARV4 and B19V DNA detection was carried out in various tissues of individuals without suspect of acute viral infection, by a real time PCR and a nested PCR, targeting the ORF2 and the ORF1 respectively. Low amount of PARV4 DNA was found frequently (>40%) in heart and liver of adults individuals, less frequently in lungs and kidneys (23,5 and 18% respectively) and was rare in bone marrow, skin and synovium samples (5,5%, 4% and 5%, respectively). By comparison, B19V DNA sequences were present in the same tissues with a higher frequency (significantly higher in myocardium, skin and bone marrow) except than in liver where the frequency was the same of PARV4 DNA and in plasma samples where B19V frequency was significantly lower than that of PARV4 CONCLUSIONS: The particular tropism of PARV4 for liver and heart, here emerged, suggests to focus further studies on these tissues as possible target for viral replication and on the possible role of PARV4 infection in liver and heart diseases. Neither bone marrow nor kidney seem to be a common target of viral replication. Twit Text FOAVip Human parvovirus PARV4 DNA in tissues from adult individuals: a comparison with human parvovirus B19 (B19V) ????Virol J http://www.kidney.de/pget.php?&tw=1&pmid=20950445 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=20950445 #FOAvip English Wikipedia <ref>{{Cite pmid|20950445}}</ref> Human parvovirus PARV4 DNA in tissues from adult individuals: a comparison with human parvovirus B19 (B19V) #MMPMID20950445 Corcioli F; Zakrzewska K; Fanci R; De Giorgi V; Innocenti M; Rotellini M; Di Lollo S; Azzi A Virol J 2010[Oct]; 7 (ä): 272 PMID20950445show ga BACKGROUND: PARV4 is a new member of the Parvoviridae family not closely related to any of the known human parvoviruses. Viremia seems to be a hallmark of PARV4 infection and viral DNA persistence has been demonstrated in a few tissues. Till now, PARV4 has not been associated with any disease and its prevalence in human population has not been clearly established. This study was aimed to assess the tissue distribution and the ability to persist of PARV4 in comparison to parvovirus B19 (B19V). RESULTS: PARV4 and B19V DNA detection was carried out in various tissues of individuals without suspect of acute viral infection, by a real time PCR and a nested PCR, targeting the ORF2 and the ORF1 respectively. Low amount of PARV4 DNA was found frequently (>40%) in heart and liver of adults individuals, less frequently in lungs and kidneys (23,5 and 18% respectively) and was rare in bone marrow, skin and synovium samples (5,5%, 4% and 5%, respectively). By comparison, B19V DNA sequences were present in the same tissues with a higher frequency (significantly higher in myocardium, skin and bone marrow) except than in liver where the frequency was the same of PARV4 DNA and in plasma samples where B19V frequency was significantly lower than that of PARV4 CONCLUSIONS: The particular tropism of PARV4 for liver and heart, here emerged, suggests to focus further studies on these tissues as possible target for viral replication and on the possible role of PARV4 infection in liver and heart diseases. Neither bone marrow nor kidney seem to be a common target of viral replication. |Adult[MESH] |Aged[MESH] |Aged, 80 and over[MESH] |Bone Marrow/virology[MESH] |Carrier State/*epidemiology/virology[MESH] |DNA, Viral/genetics/*isolation & purification[MESH] |Heart/virology[MESH] |Humans[MESH] |Kidney/virology[MESH] |Liver/virology[MESH] |Lung/virology[MESH] |Middle Aged[MESH] |Open Reading Frames[MESH] |Parvoviridae Infections/*epidemiology/virology[MESH] |Parvoviridae/*classification/genetics/*isolation & purification[MESH] |Plasma/virology[MESH] |Polymerase Chain Reaction[MESH] |Skin/virology[MESH] |Synovial Membrane/virology[MESH]Human parvovirus PARV4 DNA in tissues from adult individuals: a compar(epmc).pdf DeepDyve Pubget Overpricing