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10.1128/JVI.00912-10 http://scihub22266oqcxt.onion/10.1128/JVI.00912-10 20943969!3004325!20943969     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Virol 2010 ; 84 (24): 13059-62 Nephropedia Template TP {{tp|p=20943969|t=2010. Codon optimization of human parvovirus B19 capsid genes greatly increases their expression in nonpermissive cells |pdf=|usr=}}{{20943969}} gab.com Text Codon optimization of human parvovirus B19 capsid genes greatly increases their expression in nonpermissive cells JO: J Virol http://www.kidney.de/pget.php?&tw=1&pmid=20943969 #FOAvip Parvovirus B19 (B19V) is pathogenic for humans and has an extreme tropism for human erythroid progenitors. We report cell type-specific expression of the B19V capsid genes (VP1 and VP2) and greatly increased B19V capsid protein production in nonpermissive cells by codon optimization. Codon usage limitation, rather than promoter type and the 3' untranslated region of the capsid genes, appears to be a key factor in capsid protein production in nonpermissive cells. Moreover, B19 virus-like particles were successfully generated in nonpermissive cells by transient transfection of a plasmid carrying both codon-optimized VP1 and VP2 genes. Twit Text FOAVip Codon optimization of human parvovirus B19 capsid genes greatly increases their expression in nonpermissive cells ????J Virol http://www.kidney.de/pget.php?&tw=1&pmid=20943969 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=20943969 #FOAvip English Wikipedia <ref>{{Cite pmid|20943969}}</ref> Codon optimization of human parvovirus B19 capsid genes greatly increases their expression in nonpermissive cells #MMPMID20943969 Zhi N; Wan Z; Liu X; Wong S; Kim DJ; Young NS; Kajigaya S J Virol 2010[Dec]; 84 (24): 13059-62 PMID20943969show ga Parvovirus B19 (B19V) is pathogenic for humans and has an extreme tropism for human erythroid progenitors. We report cell type-specific expression of the B19V capsid genes (VP1 and VP2) and greatly increased B19V capsid protein production in nonpermissive cells by codon optimization. Codon usage limitation, rather than promoter type and the 3' untranslated region of the capsid genes, appears to be a key factor in capsid protein production in nonpermissive cells. Moreover, B19 virus-like particles were successfully generated in nonpermissive cells by transient transfection of a plasmid carrying both codon-optimized VP1 and VP2 genes. |*Codon[MESH] |3' Untranslated Regions/genetics[MESH] |Capsid Proteins/genetics/*metabolism[MESH] |Capsid/metabolism[MESH] |Cell Nucleus/genetics/metabolism[MESH] |Cells, Cultured[MESH] |HeLa Cells[MESH] |Humans[MESH] |Immunoblotting[MESH] |Kidney/cytology/metabolism/virology[MESH] |Parvoviridae Infections/genetics/*metabolism/virology[MESH] |Parvovirus B19, Human/*genetics[MESH]Codon optimization of human parvovirus B19 capsid genes greatly increa(epmc).pdf DeepDyve Pubget Overpricing