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A natural process of cirrhosis resolution and deceleration of liver regeneration after thioacetamide withdrawal in a rat model #MMPMID20931291
Gu K; Zhao JD; Ren ZG; Ma NY; Lai ST; Wang J; Liu J; Jiang GL
Mol Biol Rep 2011[Mar]; 38 (3): 1687-96 PMID20931291show ga
Characteristics of thioacetamide (TAA)-induced liver cirrhosis in rat was observed for 120 days after TAA withdrawal as part of the radiobiological study of partial liver irradiation on TAA-induced cirrhotic rats. The natural process focused on cirrhosis and regeneration was recorded as a baseline condition for the interpretation of the outcome of the partial liver irradiation study. Cirrhosis in rats was successfully induced by drinking 0.03% TAA water orally for 29 weeks with a modeling rate of 96%. After establishment of the cirrhosis model, the rats were observed for 120 days upon TAA withdrawal to investigate the dynamic changes of cirrhosis and regeneration. The following characteristics were observed: (1) Histological changes; (2) Liver functions; (3) Cirrhosis: trichrome stain, quantification of hydroxyproline in hydrolysed liver tissue and TGF-beta1; (4) Liver regeneration: liver index, hepatocyte mitotic index (MI), hepatocyte proliferation index (PI) by flow cytometry, PCNA labeling index (LI) by IHC and expression of PCNA mRNA; and (5) Growth factors: serum HGF, VEGF, TGF-alpha, and IL-6. After TAA withdrawal, gradual improvement in liver functions was noted with decreases of ALT, AST, and ALP, and increase of PA. The resolution of cirrhosis was evident by histological improvement with attenuation of collagen fiber and decrease of TGF-beta1 IHC index, and also decrease of trichrome stain and hydroxyproline content. However, cirrhosis was still existed on 120 days after TAA withdrawal. Significant deceleration of liver regeneration was demonstrated with TAA withdrawal, evidenced by decrease of MI and PI, reduced expression of PCNA mRNA and PCNA LI. In conclusion, upon TAA withdrawal hepatic cirrhosis was continuously resolved, but persisted up to 120 days, and liver regeneration was significantly decelerated.
|Animals[MESH]
|Body Weight/drug effects[MESH]
|Collagen/metabolism[MESH]
|Disease Models, Animal[MESH]
|Hydroxyproline/metabolism[MESH]
|Immunohistochemistry[MESH]
|Intercellular Signaling Peptides and Proteins/blood[MESH]
Mol+Biol+Rep 2011 ; 38 (3): 1687-96
