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10.1002/9780471729259.mc1705s18 http://scihub22266oqcxt.onion/10.1002/9780471729259.mc1705s18 20812217!2964379!20812217     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Curr+Protoc+Microbiol 2010 ; Chapter 17 (1): Unit 17.5. Nephropedia Template TP {{tp|p=20812217|t=2010. Cell-based hepatitis C virus infection fluorescence resonance energy transfer (FRET) assay for antiviral compound screening |pdf=|usr=}}{{20812217}} gab.com Text Cell-based hepatitis C virus infection fluorescence resonance energy transfer (FRET) assay for antiviral compound screening JO: Curr Protoc Microbiol http://www.kidney.de/pget.php?&tw=1&pmid=20812217 #FOAvip Hepatitis C virus (HCV) affects an estimated 3% of the population and is a leading cause of chronic liver disease worldwide. Since HCV therapeutic and preventative options are limited, the development of new HCV antivirals has become a global health care concern. This has spurred the development of cell-based infectious HCV high-throughput screening assays to test the ability of compounds to inhibit HCV infection. This unit describes methods that may be used to assess the in vitro efficacy of HCV antivirals using a cell-based high-throughput fluorescence resonance energy transfer (FRET) HCV infection screening assay, which allows for the identification of inhibitors that target HCV at any step in the viral life cycle. Basic protocols are provided for compound screening during HCV infection and analysis of compound efficacy using an HCV FRET assay. Support protocols are provided for propagation of infectious HCV and measurement of viral infectivity. Twit Text FOAVip Cell-based hepatitis C virus infection fluorescence resonance energy transfer (FRET) assay for antiviral compound screening ????Curr Protoc Microbiol http://www.kidney.de/pget.php?&tw=1&pmid=20812217 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=20812217 #FOAvip English Wikipedia <ref>{{Cite pmid|20812217}}</ref> Cell-based hepatitis C virus infection fluorescence resonance energy transfer (FRET) assay for antiviral compound screening #MMPMID20812217 Yu X; Uprichard SL Curr Protoc Microbiol 2010[Aug]; Chapter 17 (1): Unit 17.5. PMID20812217show ga Hepatitis C virus (HCV) affects an estimated 3% of the population and is a leading cause of chronic liver disease worldwide. Since HCV therapeutic and preventative options are limited, the development of new HCV antivirals has become a global health care concern. This has spurred the development of cell-based infectious HCV high-throughput screening assays to test the ability of compounds to inhibit HCV infection. This unit describes methods that may be used to assess the in vitro efficacy of HCV antivirals using a cell-based high-throughput fluorescence resonance energy transfer (FRET) HCV infection screening assay, which allows for the identification of inhibitors that target HCV at any step in the viral life cycle. Basic protocols are provided for compound screening during HCV infection and analysis of compound efficacy using an HCV FRET assay. Support protocols are provided for propagation of infectious HCV and measurement of viral infectivity. |Antiviral Agents/*pharmacology[MESH] |Cell Line[MESH] |Drug Evaluation, Preclinical/*methods[MESH] |Fluorescence Resonance Energy Transfer/*methods[MESH] |Hepacivirus/*drug effects[MESH]Cell-based hepatitis C virus infection fluorescence resonance energy t(epmc).pdf DeepDyve Pubget Overpricing