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10.1002/btpr.480 http://scihub22266oqcxt.onion/10.1002/btpr.480 20809484!7161902!20809484     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Biotechnol+Prog 2010 ; 26 (6): 1733-40 Nephropedia Template TP {{tp|p=20809484|t=2010. Generating stable Chinese hamster ovary cell clones to produce a truncated SARS-CoV spike protein for vaccine development |pdf=|usr=}}{{20809484}} gab.com Text Generating stable Chinese hamster ovary cell clones to produce a truncated SARS-CoV spike protein for vaccine development JO: Biotechnol Prog http://www.kidney.de/pget.php?&tw=1&pmid=20809484 #FOAvip The spike (S) protein of the severe acute respiratory syndrome coronavirus (SARS-CoV) is important for vaccine development. S(TR2) (an 88 kDa truncated SARS-CoV TW1 S protein carrying the S fragments S-74-253, S-294-739, and S-1129-1255) is capable of expressing a major form of glycoprotein as endo H-sensitive ( approximately 115 kDa) in CHO cells. To establish stable expressing cell clones, we transfected CHO/dhFr-cells with the amplifiable vectors ISID (IRES-driven dhfr) and ISIZ (SV40-driven dhfr) to select stepwise MTX, and observed enhanced approximately 115 kDa glycoform generation through gene amplification. Following stepwise MTX selection, we compared gene amplification levels between two vectors in engineered CHO cell chromosomes. These results confirm that the IRES-driven dhfr promoter generates greater gene amplification, which in turn enhances S(TR2) expression. Our results indicate that the approximately 115 kDa glycoform of S(TR2) protein was capable of increasing after gene amplification. The S(TR2) glycoform did not change between suspension and serum-free cultures, suggesting that the stable and amplified cell clones analyzed in this study have potential for producing homologous S(TR2) on a large scale. Twit Text FOAVip Generating stable Chinese hamster ovary cell clones to produce a truncated SARS-CoV spike protein for vaccine development ????Biotechnol Prog http://www.kidney.de/pget.php?&tw=1&pmid=20809484 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=20809484 #FOAvip English Wikipedia <ref>{{Cite pmid|20809484}}</ref> Generating stable Chinese hamster ovary cell clones to produce a truncated SARS-CoV spike protein for vaccine development #MMPMID20809484 Lin SC; Leng CH; Wu SC Biotechnol Prog 2010[Nov]; 26 (6): 1733-40 PMID20809484show ga The spike (S) protein of the severe acute respiratory syndrome coronavirus (SARS-CoV) is important for vaccine development. S(TR2) (an 88 kDa truncated SARS-CoV TW1 S protein carrying the S fragments S-74-253, S-294-739, and S-1129-1255) is capable of expressing a major form of glycoprotein as endo H-sensitive ( approximately 115 kDa) in CHO cells. To establish stable expressing cell clones, we transfected CHO/dhFr-cells with the amplifiable vectors ISID (IRES-driven dhfr) and ISIZ (SV40-driven dhfr) to select stepwise MTX, and observed enhanced approximately 115 kDa glycoform generation through gene amplification. Following stepwise MTX selection, we compared gene amplification levels between two vectors in engineered CHO cell chromosomes. These results confirm that the IRES-driven dhfr promoter generates greater gene amplification, which in turn enhances S(TR2) expression. Our results indicate that the approximately 115 kDa glycoform of S(TR2) protein was capable of increasing after gene amplification. The S(TR2) glycoform did not change between suspension and serum-free cultures, suggesting that the stable and amplified cell clones analyzed in this study have potential for producing homologous S(TR2) on a large scale. |Animals[MESH] |CHO Cells[MESH] |Clone Cells[MESH] |Cricetinae[MESH] |Cricetulus[MESH] |Membrane Glycoproteins/*biosynthesis/genetics[MESH] |Reverse Transcriptase Polymerase Chain Reaction[MESH] |Severe acute respiratory syndrome-related coronavirus/*metabolism[MESH] |Spike Glycoprotein, Coronavirus[MESH] |Viral Envelope Proteins/*biosynthesis/genetics[MESH]Generating stable Chinese hamster ovary cell clones to produce a trunc(epmc).pdf DeepDyve Pubget Overpricing