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10.1002/pmic.200900840 http://scihub22266oqcxt.onion/10.1002/pmic.200900840 20662102!3664454!20662102     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Proteomics 2010 ; 10 (17): 3190-202 Nephropedia Template TP {{tp|p=20662102|t=2010. Proteomic analysis identifies highly antigenic proteins in exosomes from M tuberculosis-infected and culture filtrate protein-treated macrophages |pdf=|usr=}}{{20662102}} gab.com Text Proteomic analysis identifies highly antigenic proteins in exosomes from M tuberculosis-infected and culture filtrate protein-treated macrophages JO: Proteomics http://www.kidney.de/pget.php?&tw=1&pmid=20662102 #FOAvip Exosomes are small 30-100 nm membrane vesicles released from hematopoietic and nonhematopoietic cells and function to promote intercellular communication. They are generated through fusion of multivesicular bodies with the plasma membrane and release of interluminal vesicles. Previous studies from our laboratory demonstrated that macrophages infected with Mycobacterium release exosomes that promote activation of both innate and acquired immune responses; however, the components present in exosomes inducing these host responses were not defined. This study used LC-MS/MS to identify 41 mycobacterial proteins present in exosomes released from M. tuberculosis-infected J774 cells. Many of these proteins have been characterized as highly immunogenic. Further, since most of the mycobacterial proteins identified are actively secreted, we hypothesized that macrophages treated with M. tuberculosis culture filtrate proteins (CFPs) would release exosomes containing mycobacterial proteins. We found 29 M. tuberculosis proteins in exosomes released from CFP-treated J774 cells, the majority of which were also present in exosomes isolated from M. tuberculosis-infected cells. The exosomes from CFP-treated J774 cells could promote macrophage and dendritic cell activation as well as activation of naive T cells in vivo. These results suggest that exosomes containing M. tuberculosis antigens may be alternative approach to developing a tuberculosis vaccine. Twit Text FOAVip Proteomic analysis identifies highly antigenic proteins in exosomes from M tuberculosis-infected and culture filtrate protein-treated macrophages ????Proteomics http://www.kidney.de/pget.php?&tw=1&pmid=20662102 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=20662102 #FOAvip English Wikipedia <ref>{{Cite pmid|20662102}}</ref> Proteomic analysis identifies highly antigenic proteins in exosomes from M tuberculosis-infected and culture filtrate protein-treated macrophages #MMPMID20662102 Giri PK; Kruh NA; Dobos KM; Schorey JS Proteomics 2010[Sep]; 10 (17): 3190-202 PMID20662102show ga Exosomes are small 30-100 nm membrane vesicles released from hematopoietic and nonhematopoietic cells and function to promote intercellular communication. They are generated through fusion of multivesicular bodies with the plasma membrane and release of interluminal vesicles. Previous studies from our laboratory demonstrated that macrophages infected with Mycobacterium release exosomes that promote activation of both innate and acquired immune responses; however, the components present in exosomes inducing these host responses were not defined. This study used LC-MS/MS to identify 41 mycobacterial proteins present in exosomes released from M. tuberculosis-infected J774 cells. Many of these proteins have been characterized as highly immunogenic. Further, since most of the mycobacterial proteins identified are actively secreted, we hypothesized that macrophages treated with M. tuberculosis culture filtrate proteins (CFPs) would release exosomes containing mycobacterial proteins. We found 29 M. tuberculosis proteins in exosomes released from CFP-treated J774 cells, the majority of which were also present in exosomes isolated from M. tuberculosis-infected cells. The exosomes from CFP-treated J774 cells could promote macrophage and dendritic cell activation as well as activation of naive T cells in vivo. These results suggest that exosomes containing M. tuberculosis antigens may be alternative approach to developing a tuberculosis vaccine. |Animals[MESH] |Antigens/*analysis/metabolism[MESH] |Blotting, Western[MESH] |Bone Marrow Cells/metabolism[MESH] |Cell Line[MESH] |Cells, Cultured[MESH] |Cytokines/metabolism[MESH] |Exosomes/*chemistry/metabolism[MESH] |Flow Cytometry[MESH] |Macrophages/immunology/metabolism/*microbiology[MESH] |Mice[MESH] |Mice, Inbred BALB C[MESH] |Mycobacterium tuberculosis/*immunology[MESH] |Proteome/analysis/metabolism[MESH] |Proteomics/*methods[MESH] |Spleen/cytology[MESH]Proteomic analysis identifies highly antigenic proteins in exosomes fr(epmc).pdf DeepDyve Pubget Overpricing