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10.1016/j.virol.2010.04.014 http://scihub22266oqcxt.onion/10.1016/j.virol.2010.04.014 20457462!2879452!20457462     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=20457462&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215       Virology 2010 ; 403 (2): 145-54 Nephropedia Template TP {{tp|p=20457462|t=2010. Characterization of the gene expression profile of human bocavirus |pdf=|usr=}}{{20457462}} gab.com Text Characterization of the gene expression profile of human bocavirus JO: Virology http://www.kidney.de/pget.php?&tw=1&pmid=20457462 #FOAvip We have generated a quantitative transcription profile of human bocavirus type 1 (HBoV1) by transfecting a nearly full-length clone in human lung epithelial A549 cells as well as in a replication competent system in 293 cells. The overall transcription profile of HBoV1 is similar to that of two other members of genus Bocavirus, minute virus of canines and bovine parvovirus 1. In particular, a spliced NS1-transcript that was not recognized previously expressed the large non-structural protein NS1 at approximately 100kDa; and the NP1-encoding transcripts were expressed abundantly. In addition, the protein expression profile of human bocavirus type 2 (HBoV2) was examined in parallel by transfection of a nearly full-length clone in A549 cells, which is similar to that of HBoV1. Moreover, our results showed that, unlike human parvovirus B19 infection, expression of the HBoV1 proteins only does not induce cell cycle arrest and apoptosis of A549 cells. Twit Text FOAVip Characterization of the gene expression profile of human bocavirus ????Virology http://www.kidney.de/pget.php?&tw=1&pmid=20457462 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=20457462 #FOAvip English Wikipedia <ref>{{Cite pmid|20457462}}</ref> Characterization of the gene expression profile of human bocavirus #MMPMID20457462 Chen AY; Cheng F; Lou S; Luo Y; Liu Z; Delwart E; Pintel D; Qiu J Virology 2010[Aug]; 403 (2): 145-54 PMID20457462show ga We have generated a quantitative transcription profile of human bocavirus type 1 (HBoV1) by transfecting a nearly full-length clone in human lung epithelial A549 cells as well as in a replication competent system in 293 cells. The overall transcription profile of HBoV1 is similar to that of two other members of genus Bocavirus, minute virus of canines and bovine parvovirus 1. In particular, a spliced NS1-transcript that was not recognized previously expressed the large non-structural protein NS1 at approximately 100kDa; and the NP1-encoding transcripts were expressed abundantly. In addition, the protein expression profile of human bocavirus type 2 (HBoV2) was examined in parallel by transfection of a nearly full-length clone in A549 cells, which is similar to that of HBoV1. Moreover, our results showed that, unlike human parvovirus B19 infection, expression of the HBoV1 proteins only does not induce cell cycle arrest and apoptosis of A549 cells. |*Gene Expression Profiling[MESH] |Bocavirus/physiology[MESH] |Cell Line[MESH] |Human bocavirus/*physiology[MESH] |Humans[MESH] |RNA Splicing[MESH] |RNA, Messenger/metabolism[MESH]Characterization of the gene expression profile of human bocavirus (epmc).pdf DeepDyve Pubget Overpricing