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10.1016/j.cyto.2010.04.001

http://scihub22266oqcxt.onion/10.1016/j.cyto.2010.04.001
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20427198!ä!20427198

suck abstract from ncbi


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pmid20427198      Cytokine 2010 ; 51 (2): 151-7
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  • Protective effects of recombinant human granulocyte macrophage colony stimulating factor on H1N1 influenza virus-induced pneumonia in mice #MMPMID20427198
  • Huang H; Li H; Zhou P; Ju D
  • Cytokine 2010[Aug]; 51 (2): 151-7 PMID20427198show ga
  • Protective effects of recombinant human granulocyte macrophage colony stimulating factor (rHuGM-CSF) on H1N1 influenza virus infection was studied in vivo and in vitro. Mice were infected with H1N1 influenza A viruses and rHuGM-CSF at doses of 0.34, 0.67, and 1.34mgkg(-1)d(-1) was administrated for 7days before the mice were infected with influenza virus and continued for a further 3days. Compared with control mice, rHuGM-CSF was demonstrated to increase the survival rate of the infected mice by 50.0%, 55.6%, and 80.0% and increased the mean survival days by 25.7%, 30.0%, and 46.8%, respectively. Histopathological study of the lungs in pneumonia mice found that pre-treatment with rHuGM-CSF significantly ameliorated lung injury induced by influenza virus infection. In vitro study demonstrated that when rHuGM-CSF were co-incubated with peripheral blood mononuclear cells (PBMCs), the PBMCs culture supernatant induced a dose-dependent reduction of virus-induced cytopathic effect (CPE) in Madin-Darby canine kidney (MDCK) cells in vitro. These results suggested that rHuGM-CSF might be an effective and potential protection for H1N1 influenza virus-induced pneumonia.
  • |*Influenza A Virus, H1N1 Subtype[MESH]
  • |Animals[MESH]
  • |Female[MESH]
  • |Granulocyte-Macrophage Colony-Stimulating Factor/*therapeutic use[MESH]
  • |Humans[MESH]
  • |Leukocytes, Mononuclear/virology[MESH]
  • |Lung/pathology/virology[MESH]
  • |Male[MESH]
  • |Mice[MESH]
  • |Mice, Inbred BALB C[MESH]
  • |Orthomyxoviridae Infections/*drug therapy/mortality/pathology[MESH]
  • |Pneumonia, Viral/*drug therapy/mortality/pathology[MESH]


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