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Identification of TRPM7 channels in human intestinal interstitial cells of Cajal #MMPMID19998500
Kim BJ; Park KJ; Kim HW; Choi S; Jun JY; Chang IY; Jeon JH; So I; Kim SJ
World J Gastroenterol 2009[Dec]; 15 (46): 5799-804 PMID19998500show ga
AIM: To investigate the characteristics of slow electrical waves and the presence of transient receptor potential melastatin-type 7 (TRPM7) in the human gastrointestinal (GI) tract. METHODS: Conventional microelectrode techniques were used to record intracellular electrical responses from human GI smooth muscle tissue. Immunohistochemistry was used to identify TRPM7 channels in interstitial cells of Cajal (ICCs). RESULTS: The human GI tract generated slow electrical waves and had ICCs which functioned as pacemaker cells. Flufenamic acid, a nonselective cation channel blocker, and 2-APB (2-aminoethoxydiphenyl borate) and La(3+), TRPM7 channel blockers, inhibited the slow waves. Also, TRPM7 channels were expressed in ICCs in human tissue. CONCLUSION: These results suggest that the human GI tract generates slow waves and that TRPM7 channels expressed in the ICCs may be involved in the generation of the slow waves.
|Animals[MESH]
|Anti-Inflammatory Agents/pharmacology[MESH]
|Boron Compounds/pharmacology[MESH]
|Female[MESH]
|Flufenamic Acid/pharmacology[MESH]
|Gastrointestinal Motility/physiology[MESH]
|Humans[MESH]
|Interstitial Cells of Cajal/cytology/*metabolism[MESH]
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World+J+Gastroenterol 2009 ; 15 (46): 5799-804
