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10.1007/BF02899412 http://scihub22266oqcxt.onion/10.1007/BF02899412 1980555!ä!1980555 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Virchows+Arch+B+Cell+Pathol+Incl+Mol+Pathol 1990 ; 59 (5): 257-61 Nephropedia Template TP {{tp|p=1980555|t=1990. In situ hybridization for the detection of human parvovirus B19 nucleic acid sequences in paraffin-embedded specimens |pdf=|usr=}}{{1980555}} gab.com Text In situ hybridization for the detection of human parvovirus B19 nucleic acid sequences in paraffin-embedded specimens JO: Virchows Arch B Cell Pathol Incl Mol Pathol http://www.kidney.de/pget.php?&tw=1&pmid=1980555 #FOAvip Parvovirus infection of pregnant women leading to a transplacentar infection of the fetus may result in hydrops fetalis, and ultimately in intrauterine death of the fetus. In situ hybridization with a biotinylated as well as with a 35S-labeled probe for human parvovirus B19 was performed on formalin-fixed paraffin-embedded (FFPE) tissue from a fetus suffering from non-immunologic hydrops fetalis. Histology was suggestive of viral infection probably with human parvovirus. Parvovirus DNA could be detected and precisely localized mainly in the nuclei of erythroid precursors cells within fetal blood vessels of all organs examined. There was no detection of B19 nucleic acid in parenchymal cells of the placenta or the fetal organs, nor within maternal blood cells. These findings are in agreement with the well-known properties of animal parvoviruses to replicate exclusively in proliferating cells. Taking into consideration the problems in diagnosing human parvovirus infection by light microscopy, we conclude that in situ hybridization with an appropriate non-radioactive probe is a valuable, rapid and safe complementary detection method for the diagnosis and study of human parvovirus infections. The 35S-labeled probe is more sensitive than the biotinylated probe, but has the disadvantages of lower resolution of the signal, longer duration of the assay, the hazard of radioactivity and the shorter shelf-life of the probe. Twit Text FOAVip In situ hybridization for the detection of human parvovirus B19 nucleic acid sequences in paraffin-embedded specimens ????Virchows Arch B Cell Pathol Incl Mol Pathol http://www.kidney.de/pget.php?&tw=1&pmid=1980555 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=1980555 #FOAvip English Wikipedia <ref>{{Cite pmid|1980555}}</ref> In situ hybridization for the detection of human parvovirus B19 nucleic acid sequences in paraffin-embedded specimens #MMPMID1980555 Hassam S; Briner J; Tratschin JD; Siegl G; Heitz PU Virchows Arch B Cell Pathol Incl Mol Pathol 1990[]; 59 (5): 257-61 PMID1980555show ga Parvovirus infection of pregnant women leading to a transplacentar infection of the fetus may result in hydrops fetalis, and ultimately in intrauterine death of the fetus. In situ hybridization with a biotinylated as well as with a 35S-labeled probe for human parvovirus B19 was performed on formalin-fixed paraffin-embedded (FFPE) tissue from a fetus suffering from non-immunologic hydrops fetalis. Histology was suggestive of viral infection probably with human parvovirus. Parvovirus DNA could be detected and precisely localized mainly in the nuclei of erythroid precursors cells within fetal blood vessels of all organs examined. There was no detection of B19 nucleic acid in parenchymal cells of the placenta or the fetal organs, nor within maternal blood cells. These findings are in agreement with the well-known properties of animal parvoviruses to replicate exclusively in proliferating cells. Taking into consideration the problems in diagnosing human parvovirus infection by light microscopy, we conclude that in situ hybridization with an appropriate non-radioactive probe is a valuable, rapid and safe complementary detection method for the diagnosis and study of human parvovirus infections. The 35S-labeled probe is more sensitive than the biotinylated probe, but has the disadvantages of lower resolution of the signal, longer duration of the assay, the hazard of radioactivity and the shorter shelf-life of the probe. |Adult[MESH] |DNA Probes[MESH] |DNA, Viral/*analysis[MESH] |Female[MESH] |Humans[MESH] |Hydrops Fetalis/microbiology/pathology[MESH] |Nucleic Acid Hybridization[MESH] |Paraffin[MESH] |Parvoviridae/*isolation & purification[MESH] |Placenta/microbiology/pathology[MESH] |Pregnancy[MESH]In situ hybridization for the detection of human parvovirus B19 nuclei(epmc).pdf DeepDyve Pubget Overpricing