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10.1128/JVI.00702-09 http://scihub22266oqcxt.onion/10.1128/JVI.00702-09 19587029!2738243!19587029     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=19587029&cmd=llinks): Failed to open stream: HTTP request failed! 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The genome of human parvovirus b19 can replicate in nonpermissive cells with the help of adenovirus genes and produces infectious virus |pdf=|usr=}}{{19587029}} gab.com Text The genome of human parvovirus b19 can replicate in nonpermissive cells with the help of adenovirus genes and produces infectious virus JO: J Virol http://www.kidney.de/pget.php?&tw=1&pmid=19587029 #FOAvip Human parvovirus B19 (B19V) is a member of the genus Erythrovirus in the family Parvoviridae. In vitro, autonomous B19V replication is limited to human erythroid progenitor cells and in a small number of erythropoietin-dependent human megakaryoblastoid and erythroid leukemic cell lines. Here we report that the failure of B19V DNA replication in nonpermissive 293 cells can be overcome by adenovirus infection. More specifically, the replication of B19V DNA in the 293 cells and the production of infectious progeny virus were made possible by the presence of the adenovirus E2a, E4orf6, and VA RNA genes that emerged during the transfection of the pHelper plasmid. Using this replication system, we identified the terminal resolution site and the nonstructural protein 1 (NS1) binding site on the right terminal palindrome of the viral genome, which is composed of a minimal origin of replication spanning 67 nucleotides. Plasmids or DNA fragments containing an NS1 expression cassette and this minimal origin were able to replicate in both pHelper-transfected 293 cells and B19V-semipermissive UT7/Epo-S1 cells. Our results have important implications for our understanding of native B19V infection. Twit Text FOAVip The genome of human parvovirus b19 can replicate in nonpermissive cells with the help of adenovirus genes and produces infectious virus ????J Virol http://www.kidney.de/pget.php?&tw=1&pmid=19587029 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=19587029 #FOAvip English Wikipedia <ref>{{Cite pmid|19587029}}</ref> The genome of human parvovirus b19 can replicate in nonpermissive cells with the help of adenovirus genes and produces infectious virus #MMPMID19587029 Guan W; Wong S; Zhi N; Qiu J J Virol 2009[Sep]; 83 (18): 9541-53 PMID19587029show ga Human parvovirus B19 (B19V) is a member of the genus Erythrovirus in the family Parvoviridae. In vitro, autonomous B19V replication is limited to human erythroid progenitor cells and in a small number of erythropoietin-dependent human megakaryoblastoid and erythroid leukemic cell lines. Here we report that the failure of B19V DNA replication in nonpermissive 293 cells can be overcome by adenovirus infection. More specifically, the replication of B19V DNA in the 293 cells and the production of infectious progeny virus were made possible by the presence of the adenovirus E2a, E4orf6, and VA RNA genes that emerged during the transfection of the pHelper plasmid. Using this replication system, we identified the terminal resolution site and the nonstructural protein 1 (NS1) binding site on the right terminal palindrome of the viral genome, which is composed of a minimal origin of replication spanning 67 nucleotides. Plasmids or DNA fragments containing an NS1 expression cassette and this minimal origin were able to replicate in both pHelper-transfected 293 cells and B19V-semipermissive UT7/Epo-S1 cells. Our results have important implications for our understanding of native B19V infection. |*DNA Replication[MESH] |*Genome, Viral[MESH] |*Parvoviridae Infections[MESH] |Adenoviridae Infections[MESH] |Adenoviridae/*genetics[MESH] |Cell Line[MESH] |DNA, Viral[MESH] |Genes, Viral/*physiology[MESH] |Humans[MESH] |Parvovirus B19, Human/*genetics[MESH]The genome of human parvovirus b19 can replicate in nonpermissive cell(epmc).pdf DeepDyve Pubget Overpricing