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10.1292/jvms.71.611

http://scihub22266oqcxt.onion/10.1292/jvms.71.611
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19498287!ä!19498287

suck abstract from ncbi


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pmid19498287      J+Vet+Med+Sci 2009 ; 71 (5): 611-6
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  • Association of tumor necrosis factor-alpha with fever and pulmonary lesion score in pigs experimentally infected with swine influenza virus subtype H1N2 #MMPMID19498287
  • Kim B; Ahn KK; Ha Y; Lee YH; Kim D; Lim JH; Kim SH; Kim MY; Cho KD; Lee BH; Chae C
  • J Vet Med Sci 2009[May]; 71 (5): 611-6 PMID19498287show ga
  • The relationship between tumor necrosis factor (TNF)-alpha level, body temperature, and pulmonary lesion score was determined in 3-week-old pigs inoculated intranasally with swine influenza virus (SIV) subtype H1N2. The expression of TNF-alpha was measured in bronchoalveolar lavage (BAL) fluids by enzyme-linked immunosorbent assay and lung tissues by immunohistochemistry. In BAL fluid, TNF-alpha concentration was maximal at 1 days post-inoculation (dpi), declined markedly by 3 dpi (P<0.05) and steady thereafter. Mean rectal temperatures were above 40 degrees C for the infected groups at 1 dpi and declined markedly by 3 dpi. The body temperatures were correlated with the levels of TNF-alpha in BAL fluids from pigs experimentally infected with SIV (r(s)=0.9754, P<0.05). The pulmonary lesion scores were correlated with the means of positive cells by immunohistochemistry for TNF- alpha (r(s)=0.9949, P<0.001). The results suggest that the expression of TNF-alpha protein plays an important role in induction of pulmonary lesion and clinical sign such as fever in SIV infection.
  • |Animals[MESH]
  • |Body Temperature/immunology[MESH]
  • |Bronchoalveolar Lavage Fluid/immunology/virology[MESH]
  • |Enzyme-Linked Immunosorbent Assay/veterinary[MESH]
  • |Immunohistochemistry/veterinary[MESH]
  • |Influenza A Virus, H1N1 Subtype/genetics/*immunology[MESH]
  • |Lung Diseases/immunology/*veterinary/virology[MESH]
  • |Macrophages, Alveolar/immunology/virology[MESH]
  • |Orthomyxoviridae Infections/immunology/*veterinary/virology[MESH]
  • |Random Allocation[MESH]
  • |Reverse Transcriptase Polymerase Chain Reaction/veterinary[MESH]
  • |Swine[MESH]
  • |Swine Diseases/immunology/*virology[MESH]


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