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10.1073/pnas.0813121106 http://scihub22266oqcxt.onion/10.1073/pnas.0813121106 19289845!2663998!19289845     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Proc+Natl+Acad+Sci+U+S+A 2009 ; 106 (13): 5330-5 Nephropedia Template TP {{tp|p=19289845|t=2009. MicroRNA expression profiles predictive of human renal allograft status |pdf=|usr=}}{{19289845}} gab.com Text MicroRNA expression profiles predictive of human renal allograft status JO: Proc Natl Acad Sci U S A http://www.kidney.de/pget.php?&tw=1&pmid=19289845 #FOAvip Immune rejection of organ transplants is a life-threatening complication and is exemplified by alterations in the expression of protein-encoding genes. Because microRNAs (miRNAs) regulate the expression of genes implicated in adaptive immunity, we investigated whether acute rejection (AR) is associated with alterations in miRNA expression within allografts and whether expression profiles are diagnostic of AR and predict allograft function. Seven of 33 renal allograft biopsies (12 AR and 21 normal) were profiled using microfluidic cards containing 365 mature human miRNAs (training set), and a subset of differentially expressed miRNAs were quantified in the remaining 26 allograft biopsies (validation set). We found a strong association between intragraft expression of miRNAs and messenger RNAs (mRNAs), and that AR, and renal allograft function, could be predicted with a high level of precision using intragraft levels of miRNAs. Our investigation of miRNA expression in normal human peripheral blood mononuclear cells (PBMCs) showed that miRNAs (miR-142-5p, -155, and -223) overexpressed in AR biopsies are highly expressed in PBMCs, and that stimulation with the mitogen phytohaemagglutinin results in an increase in the abundance of miR-155 and a decrease in miR-223 and let-7c. Quantification of miRNAs in primary cultures of human renal epithelial cells (HRECs) showed that miR-30a-3p, -10b, and let-7c are highly expressed in HRECs, and that stimulation results in a decreased expression of miR-30a-3p. Our studies, in addition to suggesting a cellular basis for the altered intragraft expression of miRNAs, propose that miRNA expression patterns may serve as biomarkers of human renal allograft status. Twit Text FOAVip MicroRNA expression profiles predictive of human renal allograft status ????Proc Natl Acad Sci U S A http://www.kidney.de/pget.php?&tw=1&pmid=19289845 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=19289845 #FOAvip English Wikipedia <ref>{{Cite pmid|19289845}}</ref> MicroRNA expression profiles predictive of human renal allograft status #MMPMID19289845 Anglicheau D; Sharma VK; Ding R; Hummel A; Snopkowski C; Dadhania D; Seshan SV; Suthanthiran M Proc Natl Acad Sci U S A 2009[Mar]; 106 (13): 5330-5 PMID19289845show ga Immune rejection of organ transplants is a life-threatening complication and is exemplified by alterations in the expression of protein-encoding genes. Because microRNAs (miRNAs) regulate the expression of genes implicated in adaptive immunity, we investigated whether acute rejection (AR) is associated with alterations in miRNA expression within allografts and whether expression profiles are diagnostic of AR and predict allograft function. Seven of 33 renal allograft biopsies (12 AR and 21 normal) were profiled using microfluidic cards containing 365 mature human miRNAs (training set), and a subset of differentially expressed miRNAs were quantified in the remaining 26 allograft biopsies (validation set). We found a strong association between intragraft expression of miRNAs and messenger RNAs (mRNAs), and that AR, and renal allograft function, could be predicted with a high level of precision using intragraft levels of miRNAs. Our investigation of miRNA expression in normal human peripheral blood mononuclear cells (PBMCs) showed that miRNAs (miR-142-5p, -155, and -223) overexpressed in AR biopsies are highly expressed in PBMCs, and that stimulation with the mitogen phytohaemagglutinin results in an increase in the abundance of miR-155 and a decrease in miR-223 and let-7c. Quantification of miRNAs in primary cultures of human renal epithelial cells (HRECs) showed that miR-30a-3p, -10b, and let-7c are highly expressed in HRECs, and that stimulation results in a decreased expression of miR-30a-3p. Our studies, in addition to suggesting a cellular basis for the altered intragraft expression of miRNAs, propose that miRNA expression patterns may serve as biomarkers of human renal allograft status. |*Gene Expression Profiling[MESH] |Artificial Intelligence[MESH] |Biomarkers[MESH] |Blood Cells[MESH] |Graft Rejection/*diagnosis[MESH] |Humans[MESH] |Kidney Transplantation/*immunology[MESH] |Leukocytes, Mononuclear[MESH] |MicroRNAs/*analysis[MESH]MicroRNA expression profiles predictive of human renal allograft statu(epmc).pdf DeepDyve Pubget Overpricing