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10.1021/cb800313m http://scihub22266oqcxt.onion/10.1021/cb800313m 19278238!2777746!19278238     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       ACS+Chem+Biol 2009 ; 4 (4): 299-307 Nephropedia Template TP {{tp|p=19278238|t=2009. Small molecule microarrays of RNA-focused peptoids help identify inhibitors of a pathogenic group I intron |pdf=|usr=}}{{19278238}} gab.com Text Small molecule microarrays of RNA-focused peptoids help identify inhibitors of a pathogenic group I intron JO: ACS Chem Biol http://www.kidney.de/pget.php?&tw=1&pmid=19278238 #FOAvip Peptoids that inhibit the group I intron RNA from Candida albicans, an opportunistic pathogen that kills immunocompromised hosts, have been identified using microarrays. The arrayed peptoid library was constructed using submonomers with moieties similar to ones found in small molecules known to bind RNA. Library members that passed quality control analysis were spotted onto a microarray and screened for binding to the C. albicans group I intron ribozyme. Each ligand binder identified from microarray-based screening inhibited self-splicing in the presence of 1 mM nucleotide concentration of bulk yeast tRNA with IC(50)'s between 150 and 2200 microM. The binding signals and the corresponding IC(50)'s were used to identify features in the peptoids that predispose them for RNA binding. After statistical analysis of the peptoids' structures that bind, a second generation of inhibitors was constructed using these important features; all second generation inhibitors have improved potencies with IC(50)'s of <100 microM. The most potent inhibitor is composed of one phenylguanidine and three tryptamine submonomers and has an IC(50) of 31 microM. This compound is 6-fold more potent than pentamidine, a clinically used drug that inhibits self-splicing. These results show that (i) modulators of RNA function can be identified by designing RNA-focused chemical libraries and screening them via microarray; (ii) statistical analysis of ligand binders can identify features in leads that predispose them for binding to their targets; and (iii) features can then be programmed into second generation inhibitors to design ligands with improved potencies. Twit Text FOAVip Small molecule microarrays of RNA-focused peptoids help identify inhibitors of a pathogenic group I intron ????ACS Chem Biol http://www.kidney.de/pget.php?&tw=1&pmid=19278238 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=19278238 #FOAvip English Wikipedia <ref>{{Cite pmid|19278238}}</ref> Small molecule microarrays of RNA-focused peptoids help identify inhibitors of a pathogenic group I intron #MMPMID19278238 Labuda LP; Pushechnikov A; Disney MD ACS Chem Biol 2009[Apr]; 4 (4): 299-307 PMID19278238show ga Peptoids that inhibit the group I intron RNA from Candida albicans, an opportunistic pathogen that kills immunocompromised hosts, have been identified using microarrays. The arrayed peptoid library was constructed using submonomers with moieties similar to ones found in small molecules known to bind RNA. Library members that passed quality control analysis were spotted onto a microarray and screened for binding to the C. albicans group I intron ribozyme. Each ligand binder identified from microarray-based screening inhibited self-splicing in the presence of 1 mM nucleotide concentration of bulk yeast tRNA with IC(50)'s between 150 and 2200 microM. The binding signals and the corresponding IC(50)'s were used to identify features in the peptoids that predispose them for RNA binding. After statistical analysis of the peptoids' structures that bind, a second generation of inhibitors was constructed using these important features; all second generation inhibitors have improved potencies with IC(50)'s of <100 microM. The most potent inhibitor is composed of one phenylguanidine and three tryptamine submonomers and has an IC(50) of 31 microM. This compound is 6-fold more potent than pentamidine, a clinically used drug that inhibits self-splicing. These results show that (i) modulators of RNA function can be identified by designing RNA-focused chemical libraries and screening them via microarray; (ii) statistical analysis of ligand binders can identify features in leads that predispose them for binding to their targets; and (iii) features can then be programmed into second generation inhibitors to design ligands with improved potencies. |Animals[MESH] |Binding Sites[MESH] |Candida albicans/*drug effects/enzymology/pathogenicity[MESH] |Dose-Response Relationship, Drug[MESH] |Introns/*genetics[MESH] |Ligands[MESH] |Molecular Conformation[MESH] |Oligonucleotide Array Sequence Analysis/*methods[MESH] |Peptide Library[MESH] |Peptoids/analogs & derivatives/chemistry/*pharmacology[MESH] |Pneumocystis carinii/drug effects/genetics[MESH] |RNA Splicing/drug effects[MESH] |RNA, Catalytic/*antagonists & inhibitors/chemistry/genetics[MESH] |RNA, Fungal/*antagonists & inhibitors/chemistry/genetics[MESH] |RNA, Transfer/*antagonists & inhibitors/chemistry/genetics[MESH]Small molecule microarrays of RNA-focused peptoids help identify inhib(epmc).pdf DeepDyve Pubget Overpricing