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10.1007/s11095-009-9839-9 http://scihub22266oqcxt.onion/10.1007/s11095-009-9839-9 19191011!?!19191011 Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=19191011&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215       Pharm+Res 2009 ; 26 (5): 1181-91 Nephropedia Template TP {{tp|p=19191011|t=2009. Highly efficient transfection of rat cortical neurons using carbosilane dendrimers unveils a neuroprotective role for HIF-1alpha in early chemical hypoxia-mediated neurotoxicity |pdf=|usr=}}{{19191011}} gab.com Text Highly efficient transfection of rat cortical neurons using carbosilane dendrimers unveils a neuroprotective role for HIF-1alpha in early chemical hypoxia-mediated neurotoxicity JO: Pharm Res http://www.kidney.de/pget.php?&tw=1&pmid=19191011 #FOAvip PURPOSE: To study the effect of a non-viral vector (carbosilane dendrimer) to efficiently deliver small interfering RNA to postmitotic neurons to study the function of hypoxia-inducible factor-1alpha (HIF1-alpha) during chemical hypoxia-mediated neurotoxicity. METHODS: Chemical hypoxia was induced in primary rat cortical neurons by exposure to CoCl(2). HIF1-alpha levels were determined by Western Blot and toxicity was evaluated by both MTT and LDH assays. Neurons were incubated with dendriplexes containing anti-HIF1-alpha siRNA and both uptake and HIF1-alpha knockdown efficiency were evaluated. RESULTS: We report that a non-viral vector (carbosilane dendrimer) can deliver specific siRNA to neurons and selectively block HIF1-alpha synthesis with similar efficiency to that achieved by viral vectors. Using this method, we have found that this transcription factor plays a neuroprotective role during the early phase of chemical hypoxia-mediated neurotoxicity. CONCLUSION: This work represents a proof-of-concept for the use of carbosilane dendrimers to deliver specific siRNA to postmitotic neurons to block selected protein synthesis. This indicates that this type of vector is a good alternative to viral vectors to achieve very high transfection levels in neurons. This also suggests that carbosilane dendrimers might be very useful for gene therapy. Twit Text FOAVip Highly efficient transfection of rat cortical neurons using carbosilane dendrimers unveils a neuroprotective role for HIF-1alpha in early chemical hypoxia-mediated neurotoxicity ????Pharm Res http://www.kidney.de/pget.php?&tw=1&pmid=19191011 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=19191011 #FOAvip English Wikipedia <ref>{{Cite pmid|19191011}}</ref> Highly efficient transfection of rat cortical neurons using carbosilane dendrimers unveils a neuroprotective role for HIF-1alpha in early chemical hypoxia-mediated neurotoxicity #MMPMID19191011 Posadas I; Lopez-Hernandez B; Clemente MI; Jimenez JL; Ortega P; de la Mata J; Gomez R; Munoz-Fernandez MA; Cena V Pharm Res 2009[May]; 26 (5): 1181-91 PMID19191011show ga PURPOSE: To study the effect of a non-viral vector (carbosilane dendrimer) to efficiently deliver small interfering RNA to postmitotic neurons to study the function of hypoxia-inducible factor-1alpha (HIF1-alpha) during chemical hypoxia-mediated neurotoxicity. METHODS: Chemical hypoxia was induced in primary rat cortical neurons by exposure to CoCl(2). HIF1-alpha levels were determined by Western Blot and toxicity was evaluated by both MTT and LDH assays. Neurons were incubated with dendriplexes containing anti-HIF1-alpha siRNA and both uptake and HIF1-alpha knockdown efficiency were evaluated. RESULTS: We report that a non-viral vector (carbosilane dendrimer) can deliver specific siRNA to neurons and selectively block HIF1-alpha synthesis with similar efficiency to that achieved by viral vectors. Using this method, we have found that this transcription factor plays a neuroprotective role during the early phase of chemical hypoxia-mediated neurotoxicity. CONCLUSION: This work represents a proof-of-concept for the use of carbosilane dendrimers to deliver specific siRNA to postmitotic neurons to block selected protein synthesis. This indicates that this type of vector is a good alternative to viral vectors to achieve very high transfection levels in neurons. This also suggests that carbosilane dendrimers might be very useful for gene therapy. |Animals[MESH] |Cell Hypoxia/drug effects[MESH] |Cell Survival/drug effects[MESH] |Cerebral Cortex/cytology[MESH] |Cobalt[MESH] |Dendrimers/chemistry/*toxicity[MESH] |Gene Knockdown Techniques[MESH] |Genetic Therapy[MESH] |Hypoxia-Inducible Factor 1, alpha Subunit/*genetics[MESH] |Hypoxia/chemically induced/*genetics/*therapy[MESH] |Neurons/*drug effects/metabolism[MESH] |Neuroprotective Agents/administration & dosage/pharmacology[MESH] |RNA, Small Interfering/*administration & dosage/genetics/pharmacology[MESH] |Rats[MESH] |Rats, Sprague-Dawley[MESH] |Silanes/chemistry/*toxicity[MESH]Highly efficient transfection of rat cortical neurons using carbosilan(epmc).pdf DeepDyve Pubget Overpricing