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http://scihub22266oqcxt.onion/ 19114343!?!19114343 Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=19114343&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215 Warning: Undefined variable $yww in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 538 Warning: Undefined variable $yww in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 538       Nan+Fang+Yi+Ke+Da+Xue+Xue+Bao 2008 ; 28 (12): 2150-3 Nephropedia Template TP {{tp|p=19114343|t=2008. Expression of TRPM and TRPV channel family mRNA in rat spermatogenic cells |pdf=|usr=}}{{19114343}} gab.com Text Expression of TRPM and TRPV channel family mRNA in rat spermatogenic cells JO: Nan Fang Yi Ke Da Xue Xue Bao http://www.kidney.de/pget.php?&tw=1&pmid=19114343 #FOAvip OBJECTIVE: To investigate the expression of transient receptor potential melastatin (TRPM) and transient receptor potential vanilloid (TRPV) channel family genes in rat spermatogenic cells. METHODS: Rat spermatogenic cells were isolated by a mechanical procedure and the total RNA was extracted using TRIzol reagent. TRPM and TRPV channel family genes were amplified by RT-PCR and the presence of the target genes was detected by agarose gel electrophoresis. The relative gene expression levels were measured by real-time quantitative RT-PCR. RESULTS: TRPV5, TRPM3, TRPM4 and TRPM7 mRNAs were expressed in rat spermatogenic cells, but TRPV1, TRPV2, TRPV3, TRPV4, TRPV6, TRPM1, TRPM2, TRPM5, TRPM6, TRPM7 and TRPM8 mRNAs were not detected. The relative expressions of TRPM and TRPV mRNA were determined by quantitative real-time RT-PCR. TRPM7 expression was the highest among all the TRPM subtypes in rat spermatogenic cells, at a level equivalent to (0.0430-/+0.0034)% of beta-actin expression. TRPM3 and TRPM4 were also highly expressed, but their expression levels were only approximately 56% and 63% of that of TRPM7, respectively. For the TRPV subfamily, only TRPV5 mRNA was abundantly expressed at the level of (0.0157-/+0.0029)% relative to that of beta-actin. CONCLUSION: TRPV5, TRPM3, TRPM4 and TRPM7 mRNAs were coexpressed in spermatogenic cells in rats, among which TRPM4 and TRPM7 mRNA were expressed at high levels. TRPM4 and TRPM7 channels may be involved in the regulation of growth, differentiation and maturation of rat spermatogenic cells and are associated with the generation of the sperms. Twit Text FOAVip Expression of TRPM and TRPV channel family mRNA in rat spermatogenic cells ????Nan Fang Yi Ke Da Xue Xue Bao http://www.kidney.de/pget.php?&tw=1&pmid=19114343 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=19114343 #FOAvip English Wikipedia <ref>{{Cite pmid|19114343}}</ref> Expression of TRPM and TRPV channel family mRNA in rat spermatogenic cells #MMPMID19114343 Li SL; Wang XH; Wang HP; Yang ZH; Gao WC; Pu XY Nan Fang Yi Ke Da Xue Xue Bao 2008[Dec]; 28 (12): 2150-3 PMID19114343show ga OBJECTIVE: To investigate the expression of transient receptor potential melastatin (TRPM) and transient receptor potential vanilloid (TRPV) channel family genes in rat spermatogenic cells. METHODS: Rat spermatogenic cells were isolated by a mechanical procedure and the total RNA was extracted using TRIzol reagent. TRPM and TRPV channel family genes were amplified by RT-PCR and the presence of the target genes was detected by agarose gel electrophoresis. The relative gene expression levels were measured by real-time quantitative RT-PCR. RESULTS: TRPV5, TRPM3, TRPM4 and TRPM7 mRNAs were expressed in rat spermatogenic cells, but TRPV1, TRPV2, TRPV3, TRPV4, TRPV6, TRPM1, TRPM2, TRPM5, TRPM6, TRPM7 and TRPM8 mRNAs were not detected. The relative expressions of TRPM and TRPV mRNA were determined by quantitative real-time RT-PCR. TRPM7 expression was the highest among all the TRPM subtypes in rat spermatogenic cells, at a level equivalent to (0.0430-/+0.0034)% of beta-actin expression. TRPM3 and TRPM4 were also highly expressed, but their expression levels were only approximately 56% and 63% of that of TRPM7, respectively. For the TRPV subfamily, only TRPV5 mRNA was abundantly expressed at the level of (0.0157-/+0.0029)% relative to that of beta-actin. CONCLUSION: TRPV5, TRPM3, TRPM4 and TRPM7 mRNAs were coexpressed in spermatogenic cells in rats, among which TRPM4 and TRPM7 mRNA were expressed at high levels. TRPM4 and TRPM7 channels may be involved in the regulation of growth, differentiation and maturation of rat spermatogenic cells and are associated with the generation of the sperms. |Animals[MESH] |Male[MESH] |RNA, Messenger/genetics/metabolism[MESH] |Rats[MESH] |Rats, Sprague-Dawley[MESH] |Spermatocytes/cytology/*metabolism[MESH] |Spermatogonia/cytology/*metabolism[MESH] |TRPM Cation Channels/genetics/*metabolism[MESH] Expression of TRPM and TRPV channel family mRNA in rat spermatogenic (epmc).pdf DeepDyve Pubget Overpricing