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10.1099/0022-1317-72-9-2251 http://scihub22266oqcxt.onion/10.1099/0022-1317-72-9-2251 1895063!ä!1895063 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Gen+Virol 1991 ; 72 ( Pt 9) (ä): 2251-5 Nephropedia Template TP {{tp|p=1895063|t=1991. Analysis of Borna disease virus-specific RNAs in infected cells and tissues |pdf=|usr=}}{{1895063}} gab.com Text Analysis of Borna disease virus-specific RNAs in infected cells and tissues JO: J Gen Virol http://www.kidney.de/pget.php?&tw=1&pmid=1895063 #FOAvip Borna disease virus (BDV) is an infectious agent that causes profound disturbances in motor function and behaviour in a wide range of animal species and possibly humans. The infectious nature of BDV has long been established, but the aetiological agent has not been isolated or classified. Recently, we have reported the isolation of BDV-specific cDNA clones using subtractive libraries constructed from mRNA from infected material. Here we describe studies on one of these cDNA clones, B8, and confirm its specificity by in situ hybridization on sections of BDV-infected brain. The complete nucleotide sequence of BDV-specific clone B8 was determined. Oligonucleotides of positive and negative polarity synthesized from sequences from the 5' and 3' ends, as well as the central part, of clone B8 identified both positive- and negative-strand BDV-specific RNAs in infected rat brain. All B8 sequences used as oligonucleotide probes were found to be contained in the larger positive- and negative-strand RNAs. Thus, the structure of the BDV-specific RNAs appears to be a nested set of multiple, overlapping subgenomic positive- and negative-strand RNA transcripts. Twit Text FOAVip Analysis of Borna disease virus-specific RNAs in infected cells and tissues ????J Gen Virol http://www.kidney.de/pget.php?&tw=1&pmid=1895063 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=1895063 #FOAvip English Wikipedia <ref>{{Cite pmid|1895063}}</ref> Analysis of Borna disease virus-specific RNAs in infected cells and tissues #MMPMID1895063 Richt JA; Vande Woude S; Zink MC; Narayan O; Clements JE J Gen Virol 1991[Sep]; 72 ( Pt 9) (ä): 2251-5 PMID1895063show ga Borna disease virus (BDV) is an infectious agent that causes profound disturbances in motor function and behaviour in a wide range of animal species and possibly humans. The infectious nature of BDV has long been established, but the aetiological agent has not been isolated or classified. Recently, we have reported the isolation of BDV-specific cDNA clones using subtractive libraries constructed from mRNA from infected material. Here we describe studies on one of these cDNA clones, B8, and confirm its specificity by in situ hybridization on sections of BDV-infected brain. The complete nucleotide sequence of BDV-specific clone B8 was determined. Oligonucleotides of positive and negative polarity synthesized from sequences from the 5' and 3' ends, as well as the central part, of clone B8 identified both positive- and negative-strand BDV-specific RNAs in infected rat brain. All B8 sequences used as oligonucleotide probes were found to be contained in the larger positive- and negative-strand RNAs. Thus, the structure of the BDV-specific RNAs appears to be a nested set of multiple, overlapping subgenomic positive- and negative-strand RNA transcripts. |Amino Acid Sequence[MESH] |Animals[MESH] |Base Sequence[MESH] |Borna disease virus/*genetics[MESH] |Brain/*microbiology[MESH] |Cloning, Molecular[MESH] |Codon/chemistry[MESH] |Molecular Sequence Data[MESH] |Nucleic Acid Hybridization[MESH] |Oligonucleotide Probes[MESH] |Open Reading Frames[MESH] |Protein Biosynthesis[MESH]Analysis of Borna disease virus-specific RNAs in infected cells and ti(epmc).pdf DeepDyve Pubget Overpricing