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10.1152/ajprenal.90229.2008 http://scihub22266oqcxt.onion/10.1152/ajprenal.90229.2008 18768590!2584897!18768590     free     free     free Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Am+J+Physiol+Renal+Physiol 2008 ; 295 (5): F1472-84 Nephropedia Template TP {{tp|p=18768590|t=2008. WNK3 positively regulates epithelial calcium channels TRPV5 and TRPV6 via a kinase-dependent pathway |pdf=|usr=}}{{18768590}} gab.com Text WNK3 positively regulates epithelial calcium channels TRPV5 and TRPV6 via a kinase-dependent pathway JO: Am J Physiol Renal Physiol http://www.kidney.de/pget.php?&tw=1&pmid=18768590 #FOAvip WNK3, a member of the With No Lysine (K) family of protein serine/threonine kinases, was shown to regulate members of the SLC12A family of cation-chloride cotransporters and the renal outer medullary K+ channel ROMK and Cl(-) channel SLC26A9. To evaluate the effect of WNK3 on TRPV5, a renal epithelial Ca2+ channel that serves as a gatekeeper for active Ca2+ reabsorption, WNK3 and TRPV5 were coexpressed in Xenopus laevis oocytes and the function and expression of TRPV5 were subsequently examined. An 82.7 +/- 7.1% increase in TRPV5-mediated Ca2+ uptake was observed when WNK3 was coexpressed. A similar increase in TRPV5-mediated Na+ current was observed with the voltage-clamp technique. WNK3 also enhanced Ca2+ influx and Na+ current mediated by TRPV6, which is the closest homolog of TRPV5 that mediates active intestinal Ca2+ absorption. The kinase domain of WNK3 alone was sufficient to increase TRPV5-mediated Ca2+ transport, and the positive regulatory effect was abolished by the kinase-inactive D294A mutation in WNK3, indicating a kinase-dependent mechanism. The complexly glycosylated TRPV5 that appears at the plasma membrane was increased by WNK3. The exocytosis of TRPV5 was increased by WNK3, and the effect of WNK3 on TRPV5 was abolished by the microtubule inhibitor colchicine. The increased plasma membrane expression of TRPV5 was likely due to the enhanced delivery of mature TRPV5 to the plasma membrane from its intracellular pool via the secretory pathway. These results indicate that WNK3 is a positive regulator of the transcellular Ca2+ transport pathway. Twit Text FOAVip WNK3 positively regulates epithelial calcium channels TRPV5 and TRPV6 via a kinase-dependent pathway ????Am J Physiol Renal Physiol http://www.kidney.de/pget.php?&tw=1&pmid=18768590 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=18768590 #FOAvip English Wikipedia <ref>{{Cite pmid|18768590}}</ref> WNK3 positively regulates epithelial calcium channels TRPV5 and TRPV6 via a kinase-dependent pathway #MMPMID18768590 Zhang W; Na T; Peng JB Am J Physiol Renal Physiol 2008[Nov]; 295 (5): F1472-84 PMID18768590show ga WNK3, a member of the With No Lysine (K) family of protein serine/threonine kinases, was shown to regulate members of the SLC12A family of cation-chloride cotransporters and the renal outer medullary K+ channel ROMK and Cl(-) channel SLC26A9. To evaluate the effect of WNK3 on TRPV5, a renal epithelial Ca2+ channel that serves as a gatekeeper for active Ca2+ reabsorption, WNK3 and TRPV5 were coexpressed in Xenopus laevis oocytes and the function and expression of TRPV5 were subsequently examined. An 82.7 +/- 7.1% increase in TRPV5-mediated Ca2+ uptake was observed when WNK3 was coexpressed. A similar increase in TRPV5-mediated Na+ current was observed with the voltage-clamp technique. WNK3 also enhanced Ca2+ influx and Na+ current mediated by TRPV6, which is the closest homolog of TRPV5 that mediates active intestinal Ca2+ absorption. The kinase domain of WNK3 alone was sufficient to increase TRPV5-mediated Ca2+ transport, and the positive regulatory effect was abolished by the kinase-inactive D294A mutation in WNK3, indicating a kinase-dependent mechanism. The complexly glycosylated TRPV5 that appears at the plasma membrane was increased by WNK3. The exocytosis of TRPV5 was increased by WNK3, and the effect of WNK3 on TRPV5 was abolished by the microtubule inhibitor colchicine. The increased plasma membrane expression of TRPV5 was likely due to the enhanced delivery of mature TRPV5 to the plasma membrane from its intracellular pool via the secretory pathway. These results indicate that WNK3 is a positive regulator of the transcellular Ca2+ transport pathway. |Amino Acid Substitution[MESH] |Animals[MESH] |Calcium Channels/genetics/*physiology[MESH] |Calcium/metabolism[MESH] |Cell Size[MESH] |Colchicine/pharmacology[MESH] |Cytochalasin D/pharmacology[MESH] |Electrophysiology[MESH] |Exocytosis/drug effects/physiology[MESH] |Female[MESH] |Glycosylation[MESH] |Humans[MESH] |Membrane Potentials/drug effects[MESH] |Oocytes/cytology/metabolism/physiology[MESH] |Phosphotransferases/genetics/*metabolism[MESH] |Protein Serine-Threonine Kinases/genetics/*metabolism[MESH] |Protein Transport/drug effects/physiology[MESH] |Sequence Deletion[MESH] |Sodium Chloride Symporters/genetics/physiology[MESH] |Sodium/metabolism[MESH] |TRPV Cation Channels/genetics/*physiology[MESH]WNK3 positively regulates epithelial calcium channels TRPV5 and TRPV6 (epmc).pdf DeepDyve Pubget Overpricing