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10.1159/000127429 http://scihub22266oqcxt.onion/10.1159/000127429 18431072!ä!18431072 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Intervirology 2008 ; 51 (2): 75-80 Nephropedia Template TP {{tp|p=18431072|t=2008. Variants of parvovirus B19: bioinformatical evaluation of nested PCR assays |pdf=|usr=}}{{18431072}} gab.com Text Variants of parvovirus B19: bioinformatical evaluation of nested PCR assays JO: Intervirology http://www.kidney.de/pget.php?&tw=1&pmid=18431072 #FOAvip Variants of parvovirus B19 are currently grouped into three genotypes: 1 (reference B19 strains), 2 and 3. It has been evidenced that isolate K71 of genotype 2 is more prevalent in skin than the conventional B19 genotype 1. In this study we investigated the detection of parvovirus B19 genotypes by using two nested PCRs and evaluating the suitability of these assays by BLAST search of parvovirus isolates. Subsequently, we analyze the present genotypes in skin biopsies. The two nested PCRs employed in this study allow to amplify 41 isolates as confirmed by bioinformatical validation. The molecular epidemiological characterization of our casistics confirmed the presence of isolate K71 in human skin. Twit Text FOAVip Variants of parvovirus B19: bioinformatical evaluation of nested PCR assays ????Intervirology http://www.kidney.de/pget.php?&tw=1&pmid=18431072 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=18431072 #FOAvip English Wikipedia <ref>{{Cite pmid|18431072}}</ref> Variants of parvovirus B19: bioinformatical evaluation of nested PCR assays #MMPMID18431072 Bergallo M; Costa C; Sidoti F; Novelli M; Ponti R; Castagnoli C; Merlino C; Bernengo MG; Cavallo R Intervirology 2008[]; 51 (2): 75-80 PMID18431072show ga Variants of parvovirus B19 are currently grouped into three genotypes: 1 (reference B19 strains), 2 and 3. It has been evidenced that isolate K71 of genotype 2 is more prevalent in skin than the conventional B19 genotype 1. In this study we investigated the detection of parvovirus B19 genotypes by using two nested PCRs and evaluating the suitability of these assays by BLAST search of parvovirus isolates. Subsequently, we analyze the present genotypes in skin biopsies. The two nested PCRs employed in this study allow to amplify 41 isolates as confirmed by bioinformatical validation. The molecular epidemiological characterization of our casistics confirmed the presence of isolate K71 in human skin. |*Genetic Variation[MESH] |*Parvovirus B19, Human/classification/genetics/isolation & purification[MESH] |Adult[MESH] |Aged[MESH] |Aged, 80 and over[MESH] |Biopsy[MESH] |Computational Biology/*methods[MESH] |DNA Primers[MESH] |DNA, Viral/analysis/isolation & purification[MESH] |Female[MESH] |Genotype[MESH] |Humans[MESH] |Lymphoma, T-Cell, Cutaneous/virology[MESH] |Male[MESH] |Middle Aged[MESH] |Parvoviridae Infections/virology[MESH] |Polymerase Chain Reaction/*methods[MESH]Variants of parvovirus B19: bioinformatical evaluation of nested PCR a(epmc).pdf DeepDyve Pubget Overpricing