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10.1002/0471142700.nc0430s27 http://scihub22266oqcxt.onion/10.1002/0471142700.nc0430s27 18428977!7162184!18428977     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=18428977&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215       Curr+Protoc+Nucleic+Acid+Chem 2007 ; Chapter 4 (1): Unit 4.30 Nephropedia Template TP {{tp|p=18428977|t=2007. Using morpholinos to control gene expression |pdf=|usr=}}{{18428977}} gab.com Text Using morpholinos to control gene expression JO: Curr Protoc Nucleic Acid Chem http://www.kidney.de/pget.php?&tw=1&pmid=18428977 #FOAvip Morpholino oligonucleotides are stable, uncharged, water-soluble molecules used to block complementary sequences of RNA, preventing processing, read-through, or protein binding at those sites. Morpholinos are typically used to block translation of mRNA and to block splicing of pre-mRNA, though they can block other interactions between biological macromolecules and RNA. Morpholinos are effective, specific, and lack non-antisense effects. They work in any cell that transcribes and translates RNA, but must be delivered into the nuclear/cytosolic compartment to be effective. Morpholinos form stable base pairs with complementary nucleic acid sequences but apparently do not bind to proteins to a significant extent. They are not recognized by any proteins and do not undergo protein-mediated catalysis; nor do they mediate RNA cleavage by RNase H or the RISC complex. This work focuses on techniques and background for using Morpholinos. Twit Text FOAVip Using morpholinos to control gene expression ????Curr Protoc Nucleic Acid Chem http://www.kidney.de/pget.php?&tw=1&pmid=18428977 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=18428977 #FOAvip English Wikipedia <ref>{{Cite pmid|18428977}}</ref> Using morpholinos to control gene expression #MMPMID18428977 Moulton JD Curr Protoc Nucleic Acid Chem 2007[Jan]; Chapter 4 (1): Unit 4.30 PMID18428977show ga Morpholino oligonucleotides are stable, uncharged, water-soluble molecules used to block complementary sequences of RNA, preventing processing, read-through, or protein binding at those sites. Morpholinos are typically used to block translation of mRNA and to block splicing of pre-mRNA, though they can block other interactions between biological macromolecules and RNA. Morpholinos are effective, specific, and lack non-antisense effects. They work in any cell that transcribes and translates RNA, but must be delivered into the nuclear/cytosolic compartment to be effective. Morpholinos form stable base pairs with complementary nucleic acid sequences but apparently do not bind to proteins to a significant extent. They are not recognized by any proteins and do not undergo protein-mediated catalysis; nor do they mediate RNA cleavage by RNase H or the RISC complex. This work focuses on techniques and background for using Morpholinos. |Animals[MESH] |Gene Expression Regulation/*drug effects[MESH] |Hydrogen Bonding[MESH] |Morpholines/*pharmacology[MESH] |Temperature[MESH]Using morpholinos to control gene expression (epmc).pdf DeepDyve Pubget Overpricing