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10.1099/vir.0.83053-0 http://scihub22266oqcxt.onion/10.1099/vir.0.83053-0 18089740!ä!18089740 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Gen+Virol 2008 ; 89 (Pt 1): 164-176 Nephropedia Template TP {{tp|p=18089740|t=2008. Simultaneous persistence of multiple genome variants of human parvovirus B19 |pdf=|usr=}}{{18089740}} gab.com Text Simultaneous persistence of multiple genome variants of human parvovirus B19 JO: J Gen Virol http://www.kidney.de/pget.php?&tw=1&pmid=18089740 #FOAvip The species human parvovirus B19 (B19V) can be divided into three genotypes. In this study, we addressed the question as to whether infection of an individual is restricted to one genotype. As viral DNA is detectable in tissue for long times after acute infection, we examined 87 liver specimens from adults for the presence of B19V DNA. Fifty-nine samples were found to be positive, 32 of them for genotype 1, 27 for genotype 2 and four for genotype 3. In four samples, DNA of two genotypes was detected; samples from three individuals were positive for genotypes 1 and 2 and a sample from one individual was positive for genotypes 1 and 3. Surprisingly, significant sequence heterogeneity was observed at approximately 1 % of the nucleotides of the genotype 1 genomes from individuals with double genotype 1 and 2 infection. Controls using different enzymes for genome amplification and dilutions of the template verified that nucleotide heterogeneity was due to the presence of three or more genome variants of genotype 1. In summary, the evidence shows that individuals can be infected with two different genotypes, and B19V DNA can persist as a population of different genomes. The results may have implications for the understanding of the antiviral immune response and the development of vaccines against B19V. Twit Text FOAVip Simultaneous persistence of multiple genome variants of human parvovirus B19 ????J Gen Virol http://www.kidney.de/pget.php?&tw=1&pmid=18089740 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=18089740 #FOAvip English Wikipedia <ref>{{Cite pmid|18089740}}</ref> Simultaneous persistence of multiple genome variants of human parvovirus B19 #MMPMID18089740 Schneider B; Hone A; Tolba RH; Fischer HP; Blumel J; Eis-Hubinger AM J Gen Virol 2008[Jan]; 89 (Pt 1): 164-176 PMID18089740show ga The species human parvovirus B19 (B19V) can be divided into three genotypes. In this study, we addressed the question as to whether infection of an individual is restricted to one genotype. As viral DNA is detectable in tissue for long times after acute infection, we examined 87 liver specimens from adults for the presence of B19V DNA. Fifty-nine samples were found to be positive, 32 of them for genotype 1, 27 for genotype 2 and four for genotype 3. In four samples, DNA of two genotypes was detected; samples from three individuals were positive for genotypes 1 and 2 and a sample from one individual was positive for genotypes 1 and 3. Surprisingly, significant sequence heterogeneity was observed at approximately 1 % of the nucleotides of the genotype 1 genomes from individuals with double genotype 1 and 2 infection. Controls using different enzymes for genome amplification and dilutions of the template verified that nucleotide heterogeneity was due to the presence of three or more genome variants of genotype 1. In summary, the evidence shows that individuals can be infected with two different genotypes, and B19V DNA can persist as a population of different genomes. The results may have implications for the understanding of the antiviral immune response and the development of vaccines against B19V. |*Genetic Variation[MESH] |*Genome, Viral[MESH] |Antibodies, Viral/genetics[MESH] |Cloning, Molecular[MESH] |DNA, Viral/*genetics[MESH] |Genotype[MESH] |Hepatectomy[MESH] |Humans[MESH] |Liver Transplantation[MESH] |Liver/*virology[MESH] |Molecular Sequence Data[MESH] |Parvovirus B19, Human/*classification/*genetics[MESH] |Phylogeny[MESH] |Polymerase Chain Reaction[MESH] |Promoter Regions, Genetic[MESH]Simultaneous persistence of multiple genome variants of human parvovir(epmc).pdf DeepDyve Pubget Overpricing