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10.1128/jcm.29.9.2047-2050.1991

http://scihub22266oqcxt.onion/10.1128/jcm.29.9.2047-2050.1991
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1774333!270258!1774333
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suck abstract from ncbi

pmid1774333      J+Clin+Microbiol 1991 ; 29 (9): 2047-50
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  • Chemiluminescence dot blot hybridization assay for detection of B19 parvovirus DNA in human sera #MMPMID1774333
  • Musiani M; Zerbini M; Gibellini D; Gentilomi G; Venturoli S; Gallinella G; Ferri E; Girotti S
  • J Clin Microbiol 1991[Sep]; 29 (9): 2047-50 PMID1774333show ga
  • A chemiluminescence dot blot hybridization assay was used for the detection of B19 parvovirus DNA in human sera by using digoxigenin-labeled probes. The probes were revealed immunoenzymatically by use of anti-digoxigenin Fab fragments conjugated with alkaline phosphatase. The chemiluminescence signal was obtained by reacting the labeled probe-target complex with an enzyme-triggerable dioxetane substrate. The emitted photons were detected with instant photographic films. In the search for B19 parvovirus DNA, 2,808 serum samples were analyzed.
  • |DNA Probes[MESH]
  • |DNA, Viral/*blood/genetics[MESH]
  • |Digoxigenin[MESH]
  • |Erythema Infectiosum/*diagnosis/microbiology[MESH]
  • |Humans[MESH]
  • |Immunoblotting/*methods/statistics & numerical data[MESH]
  • |Luminescent Measurements[MESH]
  • |Nucleic Acid Hybridization[MESH]
  • |Parvovirus B19, Human/genetics/*isolation & purification[MESH]


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