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10.1016/j.febslet.2007.05.045 http://scihub22266oqcxt.onion/10.1016/j.febslet.2007.05.045 17544399!ä!17544399 Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=17544399&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       FEBS+Lett 2007 ; 581 (16): 2943-6 Nephropedia Template TP {{tp|p=17544399|t=2007. A Cre-loxP based system for studying horizontal gene transfer |pdf=|usr=}}{{17544399}} gab.com Text A Cre-loxP based system for studying horizontal gene transfer JO: FEBS Lett http://www.kidney.de/pget.php?&tw=1&pmid=17544399 #FOAvip We have previously shown that DNA can be transferred to phagocytosing cells via the uptake of apoptotic cells. We report a model system that facilitates study of antigen presentation of genes transferred specifically via horizontal gene transfer. Constructs were generated encoding the LacZ gene or the influenza A nucleoprotein silenced by a STOP sequence flanked by two loxP sites. These reporter genes were demonstrated to be silent in donor cells and become activated after phagocytosis of Cre-expressing fibroblasts or macrophages. These results provide a model system for studying the influence of horizontally transferred antigens on activation of the immune system. Twit Text FOAVip A Cre-loxP based system for studying horizontal gene transfer ????FEBS Lett http://www.kidney.de/pget.php?&tw=1&pmid=17544399 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=17544399 #FOAvip English Wikipedia <ref>{{Cite pmid|17544399}}</ref> A Cre-loxP based system for studying horizontal gene transfer #MMPMID17544399 Bergsmedh A; Ehnfors J; Spetz AL; Holmgren L FEBS Lett 2007[Jun]; 581 (16): 2943-6 PMID17544399show ga We have previously shown that DNA can be transferred to phagocytosing cells via the uptake of apoptotic cells. We report a model system that facilitates study of antigen presentation of genes transferred specifically via horizontal gene transfer. Constructs were generated encoding the LacZ gene or the influenza A nucleoprotein silenced by a STOP sequence flanked by two loxP sites. These reporter genes were demonstrated to be silent in donor cells and become activated after phagocytosis of Cre-expressing fibroblasts or macrophages. These results provide a model system for studying the influence of horizontally transferred antigens on activation of the immune system. |*Gene Transfer, Horizontal[MESH] |Animals[MESH] |Cells, Cultured[MESH] |Embryo, Mammalian/cytology[MESH] |Female[MESH] |Gene Silencing[MESH] |HeLa Cells[MESH] |Humans[MESH] |Immunity, Cellular/genetics[MESH] |Influenza A virus/genetics[MESH] |Integrases/*genetics[MESH] |Lac Operon[MESH] |Mice[MESH] |Models, Genetic[MESH] |Phagocytosis/genetics[MESH] |Pregnancy[MESH] |Recombination, Genetic[MESH] |Transfection/*methods[MESH]A Cre-loxP based system for studying horizontal gene transfer (epmc).pdf DeepDyve Pubget Overpricing