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10.1128/JVI.02713-06 http://scihub22266oqcxt.onion/10.1128/JVI.02713-06 17409158!1933287!17409158     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Virol 2007 ; 81 (13): 6927-35 Nephropedia Template TP {{tp|p=17409158|t=2007. Biological and immunological relations among human parvovirus B19 genotypes 1 to 3 |pdf=|usr=}}{{17409158}} gab.com Text Biological and immunological relations among human parvovirus B19 genotypes 1 to 3 JO: J Virol http://www.kidney.de/pget.php?&tw=1&pmid=17409158 #FOAvip The human parvovirus B19 is now divided into three genotypes: type 1 (prototype), type 2 (A6- and LaLi-like), and type 3 (V9-like). In overall DNA sequence, the three virus types differ by approximately 10%. The most striking DNA dissimilarity, of >20%, is observed within the p6 promoter region. Because of the scarcity of data on the biological activities and pathogenetic potentials of virus types 2 and 3, we examined the functional characteristics of these virus types. We found the activities of the three p6 promoters to be of equal strength and to be most active in B19-permissive cells. Virus type 2 capsid protein VP2, alone or together with VP1, was expressed with the baculovirus system and was shown to assemble into icosahedral parvovirus-like particles, which were reactive in the hemagglutination assay. Furthermore, sera containing DNA of any of the three B19 types were shown to hemagglutinate. The infectivities of these sera were examined in two B19-permissive cell lines. Reverse transcription-PCR revealed synthesis of spliced B19 mRNAs, and immunofluorescence verified the production of NS and VP proteins in the infected cells. All three genotypes showed similar functional characteristics in all experiments performed, showing that the three virus types indeed belong to the same species, i.e., human parvovirus B19. Additionally, the antibody activity in sera from B19 type 1- or type 2-infected subjects (long-term immunity) was examined with homo- and heterologous virus-like particles. Cross-reactivity of 100% was observed, indicating that the two B19 genotypes comprise a single serotype. Twit Text FOAVip Biological and immunological relations among human parvovirus B19 genotypes 1 to 3 ????J Virol http://www.kidney.de/pget.php?&tw=1&pmid=17409158 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=17409158 #FOAvip English Wikipedia <ref>{{Cite pmid|17409158}}</ref> Biological and immunological relations among human parvovirus B19 genotypes 1 to 3 #MMPMID17409158 Ekman A; Hokynar K; Kakkola L; Kantola K; Hedman L; Bonden H; Gessner M; Aberham C; Norja P; Miettinen S; Hedman K; Soderlund-Venermo M J Virol 2007[Jul]; 81 (13): 6927-35 PMID17409158show ga The human parvovirus B19 is now divided into three genotypes: type 1 (prototype), type 2 (A6- and LaLi-like), and type 3 (V9-like). In overall DNA sequence, the three virus types differ by approximately 10%. The most striking DNA dissimilarity, of >20%, is observed within the p6 promoter region. Because of the scarcity of data on the biological activities and pathogenetic potentials of virus types 2 and 3, we examined the functional characteristics of these virus types. We found the activities of the three p6 promoters to be of equal strength and to be most active in B19-permissive cells. Virus type 2 capsid protein VP2, alone or together with VP1, was expressed with the baculovirus system and was shown to assemble into icosahedral parvovirus-like particles, which were reactive in the hemagglutination assay. Furthermore, sera containing DNA of any of the three B19 types were shown to hemagglutinate. The infectivities of these sera were examined in two B19-permissive cell lines. Reverse transcription-PCR revealed synthesis of spliced B19 mRNAs, and immunofluorescence verified the production of NS and VP proteins in the infected cells. All three genotypes showed similar functional characteristics in all experiments performed, showing that the three virus types indeed belong to the same species, i.e., human parvovirus B19. Additionally, the antibody activity in sera from B19 type 1- or type 2-infected subjects (long-term immunity) was examined with homo- and heterologous virus-like particles. Cross-reactivity of 100% was observed, indicating that the two B19 genotypes comprise a single serotype. |Antibodies, Viral/blood/*immunology[MESH] |Baculoviridae[MESH] |Capsid Proteins/genetics/*immunology[MESH] |Capsid/*immunology[MESH] |Genotype[MESH] |HeLa Cells[MESH] |Hemagglutination/genetics/*immunology[MESH] |Humans[MESH] |Parvoviridae Infections/blood/genetics/*immunology[MESH] |Parvovirus B19, Human/genetics/*immunology[MESH] |Promoter Regions, Genetic/genetics/immunology[MESH] |Species Specificity[MESH]Biological and immunological relations among human parvovirus B19 geno(epmc).pdf DeepDyve Pubget Overpricing