Use my Search Websuite to scan PubMed, PMCentral, Journal Hosts and Journal Archives, FullText. Kick-your-searchterm to multiple Engines kick-your-query now !>

A dictionary by aggregated review articles of nephrology, medicine and the life sciences Your one-stop-run pathway from word to the immediate pdf of peer-reviewed on-topic knowledge.

10.1016/j.micinf.2006.10.015 http://scihub22266oqcxt.onion/10.1016/j.micinf.2006.10.015 17194611!7110773!17194611     free     free     free Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 253.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Microbes+Infect 2007 ; 9 (1): 96-102 Nephropedia Template TP {{tp|p=17194611|t=2007. Lipid rafts play an important role in the early stage of severe acute respiratory syndrome-coronavirus life cycle |pdf=|usr=}}{{17194611}} gab.com Text Lipid rafts play an important role in the early stage of severe acute respiratory syndrome-coronavirus life cycle JO: Microbes Infect http://www.kidney.de/pget.php?&tw=1&pmid=17194611 #FOAvip Lipid rafts are involved in the life cycle of many viruses. In this study, we showed that lipid rafts also play an important role in the life cycle of severe acute respiratory syndrome (SARS)-coronavirus (CoV). Cholesterol depletion by pretreatment of Vero E6 cells with methyl-beta-cyclodextrin (MbetaCD) inhibited the production of SARS-CoV particles released from the infected cells. This inhibition was prevented by addition of cholesterol to the culture medium, indicating that the reduction of virus particle release was caused by the loss of cholesterol in the cell membrane. In contrast, cholesterol depletion at the post-entry stage (3h post-infection) caused only a limited effect on virus particle release. Northern blot analysis revealed that the levels of viral mRNAs were significantly affected by pretreatment with MbetaCD, but not by treatment at 3h post-infection. Interestingly, no apparent evidence for colocalization of angiotensin converting enzyme 2 with lipid rafts in the membrane of Vero E6 cells was obtained. These results suggest that lipid rafts could contribute to SARS-CoV infection in the early replication process in Vero E6 cells. Twit Text FOAVip Lipid rafts play an important role in the early stage of severe acute respiratory syndrome-coronavirus life cycle ????Microbes Infect http://www.kidney.de/pget.php?&tw=1&pmid=17194611 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=17194611 #FOAvip English Wikipedia <ref>{{Cite pmid|17194611}}</ref> Lipid rafts play an important role in the early stage of severe acute respiratory syndrome-coronavirus life cycle #MMPMID17194611 Li GM; Li YG; Yamate M; Li SM; Ikuta K Microbes Infect 2007[Jan]; 9 (1): 96-102 PMID17194611show ga Lipid rafts are involved in the life cycle of many viruses. In this study, we showed that lipid rafts also play an important role in the life cycle of severe acute respiratory syndrome (SARS)-coronavirus (CoV). Cholesterol depletion by pretreatment of Vero E6 cells with methyl-beta-cyclodextrin (MbetaCD) inhibited the production of SARS-CoV particles released from the infected cells. This inhibition was prevented by addition of cholesterol to the culture medium, indicating that the reduction of virus particle release was caused by the loss of cholesterol in the cell membrane. In contrast, cholesterol depletion at the post-entry stage (3h post-infection) caused only a limited effect on virus particle release. Northern blot analysis revealed that the levels of viral mRNAs were significantly affected by pretreatment with MbetaCD, but not by treatment at 3h post-infection. Interestingly, no apparent evidence for colocalization of angiotensin converting enzyme 2 with lipid rafts in the membrane of Vero E6 cells was obtained. These results suggest that lipid rafts could contribute to SARS-CoV infection in the early replication process in Vero E6 cells. |Angiotensin-Converting Enzyme 2[MESH] |Animals[MESH] |Chlorocebus aethiops[MESH] |Cholesterol/deficiency/metabolism/pharmacology[MESH] |Flow Cytometry[MESH] |Life Cycle Stages[MESH] |Membrane Microdomains/metabolism/*virology[MESH] |Peptidyl-Dipeptidase A/metabolism[MESH] |RNA, Messenger/genetics/metabolism[MESH] |Severe Acute Respiratory Syndrome/metabolism/virology[MESH] |Severe acute respiratory syndrome-related coronavirus/genetics/growth & development/*physiology[MESH] |Vero Cells[MESH] |Virus Replication/drug effects/physiology[MESH]Lipid rafts play an important role in the early stage of severe acute (epmc).pdf DeepDyve Pubget Overpricing