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10.1016/j.jcv.2006.05.005 http://scihub22266oqcxt.onion/10.1016/j.jcv.2006.05.005 16822717!ä!16822717 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Clin+Virol 2006 ; 36 (4): 309-11 Nephropedia Template TP {{tp|p=16822717|t=2006. Failure to detect Borna disease virus antigen and RNA in human blood |pdf=|usr=}}{{16822717}} gab.com Text Failure to detect Borna disease virus antigen and RNA in human blood JO: J Clin Virol http://www.kidney.de/pget.php?&tw=1&pmid=16822717 #FOAvip BACKGROUND: Borna disease virus (BDV) is the etiological agent of a rare progressive meningoencephalitis that affects mostly horses and sheep. There is an unresolved debate whether also humans are susceptible to infection with BDV and if so, whether this might be associated with neuropsychiatric diseases. One recent key publication employing an ELISA-based sandwich assay reported prevalences of BDV-specific circulating immune complexes in human blood as high as 30% in the normal population and up to 100% in psychiatric patients [Bode L, Reckwald P, Severus WE, Stoyloff R, Ferszt R, Dietrich DE, et al. Borna disease virus-specific circulating immune complexes, antigenemia, and free antibodies--the key marker triplet determining infection and prevailing in severe mood disorders. Mol Psychiatry 2001;6(4):481-91]. However, this report did not examine for the physical presence of BDV antigens in human blood, and therefore, these seemingly high prevalences may not reflect Borna virus-specific signals. OBJECTIVES: We attempted to correlate string plasma signals in the particular sandwich ELISA system with the presence of BDV antigens. STUDY DESIGN: Four preselected plasma samples with high reactivity in the described assay were analysed by immunoaffinity purification and highly sensitive real-time RT-PCR. RESULTS: Neither method did provide any evidence for the presence of viral proteins or nucleic acids. CONCLUSIONS: Our findings argue against the concept that the described sandwich ELISA reliably detects BDV-specific antigens in human blood, therefore do not support the hypothesis that BDV is a pathogen of humans. Twit Text FOAVip Failure to detect Borna disease virus antigen and RNA in human blood ????J Clin Virol http://www.kidney.de/pget.php?&tw=1&pmid=16822717 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=16822717 #FOAvip English Wikipedia <ref>{{Cite pmid|16822717}}</ref> Failure to detect Borna disease virus antigen and RNA in human blood #MMPMID16822717 Wolff T; Heins G; Pauli G; Burger R; Kurth R J Clin Virol 2006[Aug]; 36 (4): 309-11 PMID16822717show ga BACKGROUND: Borna disease virus (BDV) is the etiological agent of a rare progressive meningoencephalitis that affects mostly horses and sheep. There is an unresolved debate whether also humans are susceptible to infection with BDV and if so, whether this might be associated with neuropsychiatric diseases. One recent key publication employing an ELISA-based sandwich assay reported prevalences of BDV-specific circulating immune complexes in human blood as high as 30% in the normal population and up to 100% in psychiatric patients [Bode L, Reckwald P, Severus WE, Stoyloff R, Ferszt R, Dietrich DE, et al. Borna disease virus-specific circulating immune complexes, antigenemia, and free antibodies--the key marker triplet determining infection and prevailing in severe mood disorders. Mol Psychiatry 2001;6(4):481-91]. However, this report did not examine for the physical presence of BDV antigens in human blood, and therefore, these seemingly high prevalences may not reflect Borna virus-specific signals. OBJECTIVES: We attempted to correlate string plasma signals in the particular sandwich ELISA system with the presence of BDV antigens. STUDY DESIGN: Four preselected plasma samples with high reactivity in the described assay were analysed by immunoaffinity purification and highly sensitive real-time RT-PCR. RESULTS: Neither method did provide any evidence for the presence of viral proteins or nucleic acids. CONCLUSIONS: Our findings argue against the concept that the described sandwich ELISA reliably detects BDV-specific antigens in human blood, therefore do not support the hypothesis that BDV is a pathogen of humans. |Antigens, Viral/*blood[MESH] |Borna disease virus/genetics/*immunology/isolation & purification[MESH] |Chromatography, Affinity/*methods[MESH] |Enzyme-Linked Immunosorbent Assay[MESH] |Humans[MESH] |RNA, Viral/blood[MESH] |Reverse Transcriptase Polymerase Chain Reaction/*methods[MESH]Failure to detect Borna disease virus antigen and RNA in human blood (epmc).pdf DeepDyve Pubget Overpricing