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10.1002/pmic.200500754 http://scihub22266oqcxt.onion/10.1002/pmic.200500754 16622835!ä!16622835 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Proteomics 2006 ; 6 (11): 3262-74 Nephropedia Template TP {{tp|p=16622835|t=2006. Comparative proteomic analysis of hypoxia-treated and untreated human leukemic U937 cells |pdf=|usr=}}{{16622835}} gab.com Text Comparative proteomic analysis of hypoxia-treated and untreated human leukemic U937 cells JO: Proteomics http://www.kidney.de/pget.php?&tw=1&pmid=16622835 #FOAvip We reported recently that moderate hypoxia and hypoxia-mimetic agents could induce growth arrest and differentiation of leukemic cells via the mediation of hypoxia-inducible factor 1 alpha (HIF-1alpha), but the exact molecular mechanisms remain largely unknown. In this study, human acute promonocytic leukemic U937 cells were incubated under 2% O2 or in 50 microM of the hypoxia mimetic agent cobalt chloride (CoCl2) and normal oxygen for 24 h, and their protein expression profiles were compared by 2-DE coupled with MALDI-TOF/TOF MS/MS. We identified 62 and 16 proteins that were significantly deregulated by hypoxia and CoCl2 treatment, respectively. These proteins were mainly involved in metabolism, gene expression regulation, signal transduction, cell proliferation, differentiation and apoptosis. As an example, N-myc downstream regulated gene 1 (NDRG1), a putative differentiation-related gene, was up-regulated in both 2% O2- and CoCl2-treated U937 cells. Moreover, enforced HIF-1alpha expression also elevated NDRG1 mRNA and protein in U937 cells. These data will provide some clues for understanding mechanisms by which leukemic cells response to hypoxia. Twit Text FOAVip Comparative proteomic analysis of hypoxia-treated and untreated human leukemic U937 cells ????Proteomics http://www.kidney.de/pget.php?&tw=1&pmid=16622835 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=16622835 #FOAvip English Wikipedia <ref>{{Cite pmid|16622835}}</ref> Comparative proteomic analysis of hypoxia-treated and untreated human leukemic U937 cells #MMPMID16622835 Han YH; Xia L; Song LP; Zheng Y; Chen WL; Zhang L; Huang Y; Chen GQ; Wang LS Proteomics 2006[Jun]; 6 (11): 3262-74 PMID16622835show ga We reported recently that moderate hypoxia and hypoxia-mimetic agents could induce growth arrest and differentiation of leukemic cells via the mediation of hypoxia-inducible factor 1 alpha (HIF-1alpha), but the exact molecular mechanisms remain largely unknown. In this study, human acute promonocytic leukemic U937 cells were incubated under 2% O2 or in 50 microM of the hypoxia mimetic agent cobalt chloride (CoCl2) and normal oxygen for 24 h, and their protein expression profiles were compared by 2-DE coupled with MALDI-TOF/TOF MS/MS. We identified 62 and 16 proteins that were significantly deregulated by hypoxia and CoCl2 treatment, respectively. These proteins were mainly involved in metabolism, gene expression regulation, signal transduction, cell proliferation, differentiation and apoptosis. As an example, N-myc downstream regulated gene 1 (NDRG1), a putative differentiation-related gene, was up-regulated in both 2% O2- and CoCl2-treated U937 cells. Moreover, enforced HIF-1alpha expression also elevated NDRG1 mRNA and protein in U937 cells. These data will provide some clues for understanding mechanisms by which leukemic cells response to hypoxia. |*Proteome[MESH] |Apoptosis/drug effects/physiology[MESH] |Cell Differentiation/drug effects/physiology[MESH] |Cell Hypoxia/drug effects/physiology[MESH] |Cell Proliferation/drug effects[MESH] |Cobalt/toxicity[MESH] |Electrophoresis, Gel, Two-Dimensional[MESH] |Gene Expression Regulation/drug effects/*physiology[MESH] |Humans[MESH] |Signal Transduction/drug effects/physiology[MESH] |Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization[MESH]Comparative proteomic analysis of hypoxia-treated and untreated human (epmc).pdf DeepDyve Pubget Overpricing