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10.1128/JVI.80.8.4114-4121.2006 http://scihub22266oqcxt.onion/10.1128/JVI.80.8.4114-4121.2006 16571827!1440431!16571827     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=16571827&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Virol 2006 ; 80 (8): 4114-21 Nephropedia Template TP {{tp|p=16571827|t=2006. Apoptosis of liver-derived cells induced by parvovirus B19 nonstructural protein |pdf=|usr=}}{{16571827}} gab.com Text Apoptosis of liver-derived cells induced by parvovirus B19 nonstructural protein JO: J Virol http://www.kidney.de/pget.php?&tw=1&pmid=16571827 #FOAvip Parvovirus B19 has been implicated in some cases of acute fulminant non-A, non-B, non-C, non-G liver failure. Our laboratory previously demonstrated that B19 infection of hepatocytes induces apoptosis and that the B19 viral nonstructural protein, NS1, may play a critical role. To study the involvement of NS1 in apoptosis of liver cells, we generated a fusion protein of NS1 with enhanced green fluorescent protein (eGFP) in a system allowing for inducible gene expression. Transfection of the liver-derived cell line HepG2 with the eGFP/NS1 vector allowed expression of the fusion protein, which was visualized by fluorescence microscopy and demonstrated by immunoblotting. The fusion protein localized to discrete domains in the nucleus. Transfection of HepG2 cells with the eGFP/NS1 vector led to apoptosis of 35% of transfected cells, a sevenfold increase over cells transfected with the parent eGFP expression vector. Mutation of the eGFP/NS1 vector to eliminate the nucleoside triphosphate-binding site of NS1 significantly decreased apoptosis, as did treatment of transfected cells with inhibitors of caspase 3 or 9. Neutralization of tumor necrosis factor alpha or Fas ligand had no effect on apoptosis. These results demonstrate that NS1 is sufficient to induce apoptosis in liver-derived cells and that it does so through the initiation of an intrinsic caspase pathway. Twit Text FOAVip Apoptosis of liver-derived cells induced by parvovirus B19 nonstructural protein ????J Virol http://www.kidney.de/pget.php?&tw=1&pmid=16571827 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=16571827 #FOAvip English Wikipedia <ref>{{Cite pmid|16571827}}</ref> Apoptosis of liver-derived cells induced by parvovirus B19 nonstructural protein #MMPMID16571827 Poole BD; Zhou J; Grote A; Schiffenbauer A; Naides SJ J Virol 2006[Apr]; 80 (8): 4114-21 PMID16571827show ga Parvovirus B19 has been implicated in some cases of acute fulminant non-A, non-B, non-C, non-G liver failure. Our laboratory previously demonstrated that B19 infection of hepatocytes induces apoptosis and that the B19 viral nonstructural protein, NS1, may play a critical role. To study the involvement of NS1 in apoptosis of liver cells, we generated a fusion protein of NS1 with enhanced green fluorescent protein (eGFP) in a system allowing for inducible gene expression. Transfection of the liver-derived cell line HepG2 with the eGFP/NS1 vector allowed expression of the fusion protein, which was visualized by fluorescence microscopy and demonstrated by immunoblotting. The fusion protein localized to discrete domains in the nucleus. Transfection of HepG2 cells with the eGFP/NS1 vector led to apoptosis of 35% of transfected cells, a sevenfold increase over cells transfected with the parent eGFP expression vector. Mutation of the eGFP/NS1 vector to eliminate the nucleoside triphosphate-binding site of NS1 significantly decreased apoptosis, as did treatment of transfected cells with inhibitors of caspase 3 or 9. Neutralization of tumor necrosis factor alpha or Fas ligand had no effect on apoptosis. These results demonstrate that NS1 is sufficient to induce apoptosis in liver-derived cells and that it does so through the initiation of an intrinsic caspase pathway. |*Apoptosis[MESH] |Amino Acid Sequence[MESH] |Caspases/physiology[MESH] |Cell Line[MESH] |DNA Damage[MESH] |Hepatocytes/*pathology[MESH] |Humans[MESH] |Molecular Sequence Data[MESH] |Parvovirus B19, Human/*pathogenicity[MESH] |Transfection[MESH]Apoptosis of liver-derived cells induced by parvovirus B19 nonstructur(epmc).pdf DeepDyve Pubget Overpricing