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10.1016/j.jcv.2006.01.011 http://scihub22266oqcxt.onion/10.1016/j.jcv.2006.01.011 16569510!?!16569510 Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=16569510&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215       J+Clin+Virol 2006 ; 36 (2): 152-5 Nephropedia Template TP {{tp|p=16569510|t=2006. Genetic variants of parvovirus B19 identified in the United Kingdom: implications for diagnostic testing |pdf=|usr=}}{{16569510}} gab.com Text Genetic variants of parvovirus B19 identified in the United Kingdom: implications for diagnostic testing JO: J Clin Virol http://www.kidney.de/pget.php?&tw=1&pmid=16569510 #FOAvip BACKGROUND: Discrepant results in diagnostic parvovirus B19 PCR assays have been observed with strains showing nucleotide sequence variation. OBJECTIVES AND STUDY DESIGN: To perform phylogenetic analysis on two parvovirus B19 strains that gave discrepant PCR results. RESULTS: One strain was found to be genotype 2; the second strain was genotype 3. CONCLUSIONS: Parvovirus B19 genotypes 2 and 3 strains were identified in diagnostic samples of UK origin following the investigation of discrepant PCR results. More structured investigations are needed to estimate the prevalence of these variants. In the meantime, diagnostic PCR results should be interpreted cautiously when they are at variance with serological testing. Manufacturers of PCR kits for the detection of B19 sequences will need to consider re-designing their primers. Twit Text FOAVip Genetic variants of parvovirus B19 identified in the United Kingdom: implications for diagnostic testing ????J Clin Virol http://www.kidney.de/pget.php?&tw=1&pmid=16569510 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=16569510 #FOAvip English Wikipedia <ref>{{Cite pmid|16569510}}</ref> Genetic variants of parvovirus B19 identified in the United Kingdom: implications for diagnostic testing #MMPMID16569510 Cohen BJ; Gandhi J; Clewley JP J Clin Virol 2006[Jun]; 36 (2): 152-5 PMID16569510show ga BACKGROUND: Discrepant results in diagnostic parvovirus B19 PCR assays have been observed with strains showing nucleotide sequence variation. OBJECTIVES AND STUDY DESIGN: To perform phylogenetic analysis on two parvovirus B19 strains that gave discrepant PCR results. RESULTS: One strain was found to be genotype 2; the second strain was genotype 3. CONCLUSIONS: Parvovirus B19 genotypes 2 and 3 strains were identified in diagnostic samples of UK origin following the investigation of discrepant PCR results. More structured investigations are needed to estimate the prevalence of these variants. In the meantime, diagnostic PCR results should be interpreted cautiously when they are at variance with serological testing. Manufacturers of PCR kits for the detection of B19 sequences will need to consider re-designing their primers. |Adult[MESH] |DNA Primers[MESH] |False Negative Reactions[MESH] |Female[MESH] |Genetic Variation[MESH] |Humans[MESH] |Male[MESH] |Middle Aged[MESH] |Parvoviridae Infections/*diagnosis/virology[MESH] |Parvovirus B19, Human/*genetics/isolation & purification[MESH] |Polymerase Chain Reaction[MESH] |Reagent Kits, Diagnostic/standards[MESH] |Species Specificity[MESH] |United Kingdom[MESH]Genetic variants of parvovirus B19 identified in the United Kingdom: i(epmc).pdf DeepDyve Pubget Overpricing