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10.1016/j.jviromet.2005.03.021

http://scihub22266oqcxt.onion/10.1016/j.jviromet.2005.03.021
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15885812!7112802!15885812
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suck abstract from ncbi


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pmid15885812      J+Virol+Methods 2005 ; 128 (1-2): 21-8
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  • Monoclonal antibodies to SARS-associated coronavirus (SARS-CoV): identification of neutralizing and antibodies reactive to S, N, M and E viral proteins #MMPMID15885812
  • Tripp RA; Haynes LM; Moore D; Anderson B; Tamin A; Harcourt BH; Jones LP; Yilla M; Babcock GJ; Greenough T; Ambrosino DM; Alvarez R; Callaway J; Cavitt S; Kamrud K; Alterson H; Smith J; Harcourt JL; Miao C; Razdan R; Comer JA; Rollin PE; Ksiazek TG; Sanchez A; Rota PA; Bellini WJ; Anderson LJ
  • J Virol Methods 2005[Sep]; 128 (1-2): 21-8 PMID15885812show ga
  • Monoclonal antibodies (Mabs) against the Urbani strain of the SARS-associated coronavirus (SARS-CoV) were developed and characterized for reactivity to SARS-CoV and SARS-CoV S, N, M, and E proteins using enzyme-linked immunoabsorbent (ELISA), radioimmunoprecipitation, immunofluorescence, Western Blot and microneutralization assays. Twenty-six mAbs were reactive to SARS-CoV by ELISA, and nine were chosen for detailed characterization. Five mAbs reacted against the S protein, two against the M protein, and one each against the N and E proteins. Two of five S protein mAbs neutralized SARS-CoV infection of Vero E6 cells and reacted to an epitope within amino acids 490-510 in the S protein. While two of the three non-neutralizing antibodies recognized at second epitope within amino acids 270-350. The mAbs characterized should prove useful for developing SARS-CoV diagnostic assays and for studying the biology of infection and pathogenesis of disease.
  • |*Antibody Specificity[MESH]
  • |Animals[MESH]
  • |Antibodies, Monoclonal/biosynthesis/*immunology[MESH]
  • |Antibodies, Viral/biosynthesis/*immunology[MESH]
  • |Cell Line[MESH]
  • |Chlorocebus aethiops[MESH]
  • |Coronavirus M Proteins[MESH]
  • |Coronavirus Nucleocapsid Proteins[MESH]
  • |Epitope Mapping[MESH]
  • |Humans[MESH]
  • |Membrane Glycoproteins/immunology[MESH]
  • |Neutralization Tests[MESH]
  • |Nucleocapsid Proteins/immunology[MESH]
  • |Severe Acute Respiratory Syndrome/virology[MESH]
  • |Severe acute respiratory syndrome-related coronavirus/*immunology[MESH]
  • |Spike Glycoprotein, Coronavirus[MESH]
  • |Vero Cells[MESH]
  • |Viral Envelope Proteins/immunology[MESH]
  • |Viral Matrix Proteins/immunology[MESH]
  • |Viral Structural Proteins/*immunology[MESH]


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