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10.1016/j.bbrc.2005.03.037 http://scihub22266oqcxt.onion/10.1016/j.bbrc.2005.03.037 15809054!ä!15809054 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\15809054.jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Biochem+Biophys+Res+Commun 2005 ; 330 (3): 701-5 Nephropedia Template TP {{tp|p=15809054|t=2005. Functional characterization of the mouse corrected solute carrier, SLC41A2 |pdf=|usr=}}{{15809054}} gab.com Text Functional characterization of the mouse corrected solute carrier, SLC41A2 JO: Biochem Biophys Res Commun http://www.kidney.de/pget.php?&tw=1&pmid=15809054 #FOAvip We have recently demonstrated that the human solute carrier, SLC41A1, is a Mg 2+ transporter that is regulated by extracellular magnesium. A BLAST search found a closely related protein encoded by SLC41A2 that may have related functional properties. In order to determine the function of SLC41A2, the corresponding cRNA was expressed in Xenopus laevis oocytes and Mg2+ currents were determined under voltage-clamp conditions. Further, real-time RT-PCR was performed to determine if SLC41A2 expression is regulated by magnesium. When expressed in oocytes, SLC41A2 mediates voltage-dependent and saturable Mg2+ uptake with a Michaelis constant of 0.34+/-0.05 mM. Expressed SLC41A2 transports a range of other divalent cations: Ba2+, Ni2+, Co2+, Fe2+, or Mn2+, but not Ca2+, Zn2+, or Cu2+. Mg2+ transport was inhibited by large concentrations of Ca2+. Real-time reverse transcription polymerase chain reaction of RNA isolated from renal distal tubule epithelial (MDCT) cells cultured in low-magnesium media relative to normal media and in kidney cortex of mice maintained on low-magnesium diets compared to those animals consuming normal diets showed that SLC41A2 transcript, unlike SLC41A1 mRNA, is not responsive to magnesium. These studies suggest that SLC41A2 is a Mg2+ transporter that might be involved in magnesium homeostasis in epithelial cells. Twit Text FOAVip Functional characterization of the mouse corrected solute carrier, SLC41A2 ????Biochem Biophys Res Commun http://www.kidney.de/pget.php?&tw=1&pmid=15809054 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=15809054 #FOAvip English Wikipedia <ref>{{Cite pmid|15809054}}</ref> Functional characterization of the mouse corrected solute carrier, SLC41A2 #MMPMID15809054 Goytain A; Quamme GA Biochem Biophys Res Commun 2005[May]; 330 (3): 701-5 PMID15809054show ga We have recently demonstrated that the human solute carrier, SLC41A1, is a Mg 2+ transporter that is regulated by extracellular magnesium. A BLAST search found a closely related protein encoded by SLC41A2 that may have related functional properties. In order to determine the function of SLC41A2, the corresponding cRNA was expressed in Xenopus laevis oocytes and Mg2+ currents were determined under voltage-clamp conditions. Further, real-time RT-PCR was performed to determine if SLC41A2 expression is regulated by magnesium. When expressed in oocytes, SLC41A2 mediates voltage-dependent and saturable Mg2+ uptake with a Michaelis constant of 0.34+/-0.05 mM. Expressed SLC41A2 transports a range of other divalent cations: Ba2+, Ni2+, Co2+, Fe2+, or Mn2+, but not Ca2+, Zn2+, or Cu2+. Mg2+ transport was inhibited by large concentrations of Ca2+. Real-time reverse transcription polymerase chain reaction of RNA isolated from renal distal tubule epithelial (MDCT) cells cultured in low-magnesium media relative to normal media and in kidney cortex of mice maintained on low-magnesium diets compared to those animals consuming normal diets showed that SLC41A2 transcript, unlike SLC41A1 mRNA, is not responsive to magnesium. These studies suggest that SLC41A2 is a Mg2+ transporter that might be involved in magnesium homeostasis in epithelial cells. |Animals[MESH] |Cation Transport Proteins/genetics/*metabolism[MESH] |Cations, Divalent/metabolism[MESH] |Gene Expression[MESH] |Humans[MESH] |Magnesium/metabolism[MESH] |Membrane Transport Proteins/genetics/*metabolism[MESH] |Mice[MESH] |Oocytes/metabolism[MESH] |Substrate Specificity[MESH]Functional characterization of the mouse corrected solute carrier, S(epmc).pdf DeepDyve Pubget Overpricing