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10.4067/s0716-97602004000300004

http://scihub22266oqcxt.onion/10.4067/s0716-97602004000300004
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15515964!ä!15515964

suck abstract from ncbi


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pmid15515964      Biol+Res 2004 ; 37 (3): 385-93
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  • Comparative effect of ion calcium and magnesium in the activation and infection of the murine macrophage by Leishmania major #MMPMID15515964
  • Lanza H; Afonso-Cardoso SR; Silva AG; Napolitano DR; Espindola FS; Pena JD; Souza MA
  • Biol Res 2004[]; 37 (3): 385-93 PMID15515964show ga
  • Amastigotes of Leishmania major have a great ability to evade destruction in host cells. This study investigated the activation in resident, inflammatory macrophages and J774 cells in vitro treated with lipopolysaccharide (LPS), soluble Leishmania antigen (SLA), calcium ionophore (CaI) and magnesium (Mg2+) alone or combined. An increase in nitric oxide (NO) production was observed in J774 or inflammatory macrophages treated with LPS alone or in combination with SLA and CaI. The same treatments did not affect the NO release by resident macrophages. There was no interference in uptake of L. major but CaI decreased intracellular proliferation of the parasite. This study demonstrated the importance of CaI in decreasing L. major proliferation inside murine macrophages while Mg2+ seemed to increase parasite proliferation. These finding may help to understand the events involved in host cells' clearance of this pathogen.
  • |Animals[MESH]
  • |Antigens, Protozoan/pharmacology[MESH]
  • |Biomarkers[MESH]
  • |Calcium/*pharmacology[MESH]
  • |Cell Culture Techniques[MESH]
  • |Female[MESH]
  • |Leishmania major/immunology/*pathogenicity[MESH]
  • |Lipopolysaccharides/pharmacology[MESH]
  • |Macrophage Activation/*drug effects/immunology[MESH]
  • |Macrophages/*parasitology[MESH]
  • |Magnesium/*pharmacology[MESH]
  • |Mice[MESH]
  • |Mice, Inbred BALB C[MESH]


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