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10.1016/j.molimm.2004.06.032 http://scihub22266oqcxt.onion/10.1016/j.molimm.2004.06.032 15488951!7112650!15488951     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Mol+Immunol 2005 ; 42 (1): 125-36 Nephropedia Template TP {{tp|p=15488951|t=2005. Molecular characterization of a panel of murine monoclonal antibodies specific for the SARS-coronavirus |pdf=|usr=}}{{15488951}} gab.com Text Molecular characterization of a panel of murine monoclonal antibodies specific for the SARS-coronavirus JO: Mol Immunol http://www.kidney.de/pget.php?&tw=1&pmid=15488951 #FOAvip The availability of monoclonal antibodies (mAbs) specific for the SARS-coronavirus (SARS-CoV) is important for the development of both diagnostic tools and treatment of infection. A molecular characterization of nine monoclonal antibodies raised in immune mice, using highly purified, inactivated SARS-CoV as the inoculating antigen, is presented in this report. These antibodies are specific for numerous viral protein targets, and six of them are able to effectively neutralize SARS-CoV in vitro, including one with a neutralizing titre of 0.075 nM. A phylogenetic analysis of the heavy and light chain sequences reveals that the mAbs share considerable homology. The majority of the heavy chains belong to a single Ig germline V-gene family, while considerably more sequence variation is evident in the light chain sequences. These analyses demonstrate that neutralization ability can be correlated with specific murine V(H)-gene alleles. For instance, one evident trend is high sequence conservation in the V(H) chains of the neutralizing mAbs, particularly in CDR-1 and CDR-2. The results suggest that optimization of murine mAbs for neutralization of SARS-CoV infection will likely be possible, and will aid in the development of diagnostic tools and passive treatments for SARS-CoV infection. Twit Text FOAVip Molecular characterization of a panel of murine monoclonal antibodies specific for the SARS-coronavirus ????Mol Immunol http://www.kidney.de/pget.php?&tw=1&pmid=15488951 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=15488951 #FOAvip English Wikipedia <ref>{{Cite pmid|15488951}}</ref> Molecular characterization of a panel of murine monoclonal antibodies specific for the SARS-coronavirus #MMPMID15488951 Gubbins MJ; Plummer FA; Yuan XY; Johnstone D; Drebot M; Andonova M; Andonov A; Berry JD Mol Immunol 2005[Jan]; 42 (1): 125-36 PMID15488951show ga The availability of monoclonal antibodies (mAbs) specific for the SARS-coronavirus (SARS-CoV) is important for the development of both diagnostic tools and treatment of infection. A molecular characterization of nine monoclonal antibodies raised in immune mice, using highly purified, inactivated SARS-CoV as the inoculating antigen, is presented in this report. These antibodies are specific for numerous viral protein targets, and six of them are able to effectively neutralize SARS-CoV in vitro, including one with a neutralizing titre of 0.075 nM. A phylogenetic analysis of the heavy and light chain sequences reveals that the mAbs share considerable homology. The majority of the heavy chains belong to a single Ig germline V-gene family, while considerably more sequence variation is evident in the light chain sequences. These analyses demonstrate that neutralization ability can be correlated with specific murine V(H)-gene alleles. For instance, one evident trend is high sequence conservation in the V(H) chains of the neutralizing mAbs, particularly in CDR-1 and CDR-2. The results suggest that optimization of murine mAbs for neutralization of SARS-CoV infection will likely be possible, and will aid in the development of diagnostic tools and passive treatments for SARS-CoV infection. |*Antibody Specificity[MESH] |Amino Acid Sequence[MESH] |Animals[MESH] |Antibodies, Monoclonal/*biosynthesis[MESH] |Evolution, Molecular[MESH] |Hybridomas[MESH] |Immunoglobulin Heavy Chains/genetics[MESH] |Immunoglobulin Light Chains/genetics[MESH] |Mice[MESH] |Molecular Sequence Data[MESH] |Neutralization Tests[MESH]Molecular characterization of a panel of murine monoclonal antibodies (epmc).pdf DeepDyve Pubget Overpricing