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10.1165/rcmb.2003-0426OC http://scihub22266oqcxt.onion/10.1165/rcmb.2003-0426OC 15256383!ä!15256383 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 269.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 269.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 269.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 269.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 269.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Am+J+Respir+Cell+Mol+Biol 2004 ; 31 (5): 544-51 Nephropedia Template TP {{tp|p=15256383|t=2004. Cobalt induces hypoxia-inducible factor-1alpha expression in airway smooth muscle cells by a reactive oxygen species- and PI3K-dependent mechanism |pdf=|usr=}}{{15256383}} gab.com Text Cobalt induces hypoxia-inducible factor-1alpha expression in airway smooth muscle cells by a reactive oxygen species- and PI3K-dependent mechanism JO: Am J Respir Cell Mol Biol http://www.kidney.de/pget.php?&tw=1&pmid=15256383 #FOAvip Cobalt can mimic hypoxia and has been implicated as a cause of lung defects. However, the effect of cobalt on airway smooth muscle (ASM) cells has not been analyzed in detail. In this article, we use primary cultures of ASM cells from rabbit trachea and show that exposure to cobalt chloride causes a rapid increase of the intracellular levels of hypoxia-inducible factor-1alpha, which is detected predominantly inside the nucleus. With the use of specific inhibitors, we demonstrate that induction of hypoxia-inducible factor-1alpha by cobalt depends on active protein synthesis but not transcription. Furthermore, wortmannin, LY294002, and N-acetyl-L-cysteine inhibit the effect of cobalt, suggesting that it involves the phosphatidylinositol 3 kinase pathway and production of reactive oxygen species. Interestingly, cobalt chloride attenuates the contractile response of rabbit airways induced by potassium chloride, but not by acetylcholine, suggesting a link between the cellular response to hypoxic stimuli and the contractile properties of ASM cells. Twit Text FOAVip Cobalt induces hypoxia-inducible factor-1alpha expression in airway smooth muscle cells by a reactive oxygen species- and PI3K-dependent mechanism ????Am J Respir Cell Mol Biol http://www.kidney.de/pget.php?&tw=1&pmid=15256383 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=15256383 #FOAvip English Wikipedia <ref>{{Cite pmid|15256383}}</ref> Cobalt induces hypoxia-inducible factor-1alpha expression in airway smooth muscle cells by a reactive oxygen species- and PI3K-dependent mechanism #MMPMID15256383 Chachami G; Simos G; Hatziefthimiou A; Bonanou S; Molyvdas PA; Paraskeva E Am J Respir Cell Mol Biol 2004[Nov]; 31 (5): 544-51 PMID15256383show ga Cobalt can mimic hypoxia and has been implicated as a cause of lung defects. However, the effect of cobalt on airway smooth muscle (ASM) cells has not been analyzed in detail. In this article, we use primary cultures of ASM cells from rabbit trachea and show that exposure to cobalt chloride causes a rapid increase of the intracellular levels of hypoxia-inducible factor-1alpha, which is detected predominantly inside the nucleus. With the use of specific inhibitors, we demonstrate that induction of hypoxia-inducible factor-1alpha by cobalt depends on active protein synthesis but not transcription. Furthermore, wortmannin, LY294002, and N-acetyl-L-cysteine inhibit the effect of cobalt, suggesting that it involves the phosphatidylinositol 3 kinase pathway and production of reactive oxygen species. Interestingly, cobalt chloride attenuates the contractile response of rabbit airways induced by potassium chloride, but not by acetylcholine, suggesting a link between the cellular response to hypoxic stimuli and the contractile properties of ASM cells. |*Reactive Oxygen Species[MESH] |Acetylcysteine/pharmacology[MESH] |Androstadienes/pharmacology[MESH] |Animals[MESH] |Blotting, Western[MESH] |Cell Nucleus/metabolism[MESH] |Chromones/pharmacology[MESH] |Cobalt/metabolism/*pharmacology[MESH] |Cycloheximide/pharmacology[MESH] |Dactinomycin/pharmacology[MESH] |Enzyme Inhibitors/pharmacology[MESH] |Hypoxia[MESH] |Hypoxia-Inducible Factor 1, alpha Subunit[MESH] |Male[MESH] |Microscopy, Fluorescence[MESH] |Models, Biological[MESH] |Morpholines/pharmacology[MESH] |Muscle, Smooth/cytology[MESH] |Phosphatidylinositol 3-Kinases/*metabolism[MESH] |Plasmids/metabolism[MESH] |Potassium Chloride/pharmacology[MESH] |Rabbits[MESH] |Time Factors[MESH] |Trachea/cytology/metabolism[MESH] |Transcription Factors/*metabolism[MESH] |Transcription, Genetic[MESH] |Transfection[MESH]Cobalt induces hypoxia-inducible factor-1alpha expression in airway sm(epmc).pdf DeepDyve Pubget Overpricing