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Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Arch+Virol 2004 ; 149 (7): 1409-14 Nephropedia Template TP
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Regulation of the Borna disease virus polymerase complex by the viral nucleoprotein p38 isoform Brief Report #MMPMID15221540
Schneider U; Naegele M; Staeheli P
Arch Virol 2004[Jul]; 149 (7): 1409-14 PMID15221540show ga
Borna disease virus (BDV) polymerase can be reconstituted by co-expression of the viral genes N, P and L. N codes for two proteins, p39 and p38, resulting from alternative translation initiation. Using a viral minireplicon system we observed that unlike p39, p38 was almost completely inactive when expressed with P and L alone. Since BDV polymerase requires a 10-20 fold excess of N protein over P for high activity, we determined whether p38 might serve as buffer that influences the viral N:P ratio. We demonstrate that p38 efficiently rescued BDV polymerase activity in cells expressing unfavorably high amounts of P.