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10.1007/s00705-004-0327-6

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suck abstract from ncbi


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pmid15221540      Arch+Virol 2004 ; 149 (7): 1409-14
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  • Regulation of the Borna disease virus polymerase complex by the viral nucleoprotein p38 isoform Brief Report #MMPMID15221540
  • Schneider U; Naegele M; Staeheli P
  • Arch Virol 2004[Jul]; 149 (7): 1409-14 PMID15221540show ga
  • Borna disease virus (BDV) polymerase can be reconstituted by co-expression of the viral genes N, P and L. N codes for two proteins, p39 and p38, resulting from alternative translation initiation. Using a viral minireplicon system we observed that unlike p39, p38 was almost completely inactive when expressed with P and L alone. Since BDV polymerase requires a 10-20 fold excess of N protein over P for high activity, we determined whether p38 might serve as buffer that influences the viral N:P ratio. We demonstrate that p38 efficiently rescued BDV polymerase activity in cells expressing unfavorably high amounts of P.
  • |Borna disease virus/*enzymology/physiology[MESH]
  • |Codon, Initiator[MESH]
  • |Gene Expression Regulation, Viral[MESH]
  • |Genes, Viral[MESH]
  • |Nucleoproteins/*genetics/*physiology[MESH]
  • |Protein Biosynthesis[MESH]
  • |Protein Isoforms/genetics/physiology[MESH]
  • |RNA-Dependent RNA Polymerase/genetics/*metabolism[MESH]
  • |Replicon[MESH]
  • |Sequence Deletion[MESH]
  • |Viral Proteins/genetics/*physiology[MESH]


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