Use my Search Websuite to scan PubMed, PMCentral, Journal Hosts and Journal Archives, FullText. Kick-your-searchterm to multiple Engines kick-your-query now !>

A dictionary by aggregated review articles of nephrology, medicine and the life sciences Your one-stop-run pathway from word to the immediate pdf of peer-reviewed on-topic knowledge.

10.1016/j.virol.2003.09.011 http://scihub22266oqcxt.onion/10.1016/j.virol.2003.09.011 14972543!ä!14972543 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Virology 2004 ; 318 (1): 142-52 Nephropedia Template TP {{tp|p=14972543|t=2004. Construction and sequencing of an infectious clone of the human parvovirus B19 |pdf=|usr=}}{{14972543}} gab.com Text Construction and sequencing of an infectious clone of the human parvovirus B19 JO: Virology http://www.kidney.de/pget.php?&tw=1&pmid=14972543 #FOAvip Human parvovirus B19 has a nonenveloped, icosahedral capsid packaging a linear single-stranded DNA genome of 5.6 kb with long inverted terminal repeats (ITR) at both the 5' and 3' end. Previous attempts to construct a full-length B19 clone were unsuccessful due to deletions in the ITR sequences. We cloned the complete parvovirus B19 genome with intact ITRs from an aplastic crisis patient. Sequence analysis of the complete viral genome indicated that both 5' and 3' ITRs have two sequence configurations and several base changes within the ITRs compared to previous published sequences. After transfection of the plasmid into permissive cells, spliced and non-spliced viral transcripts and viral capsid proteins could be detected. Southern blot analysis of the DNA purified from the plasmid-transfected cells confirmed parvovirus B19 DNA replication. Production of infectious virus by the B19 plasmid was shown by inoculation of cell lysate derived from transfected cells into fresh cells. Together, these results indicate the first successful production of an infectious clone for parvovirus B19 virus. Twit Text FOAVip Construction and sequencing of an infectious clone of the human parvovirus B19 ????Virology http://www.kidney.de/pget.php?&tw=1&pmid=14972543 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=14972543 #FOAvip English Wikipedia <ref>{{Cite pmid|14972543}}</ref> Construction and sequencing of an infectious clone of the human parvovirus B19 #MMPMID14972543 Zhi N; Zadori Z; Brown KE; Tijssen P Virology 2004[Jan]; 318 (1): 142-52 PMID14972543show ga Human parvovirus B19 has a nonenveloped, icosahedral capsid packaging a linear single-stranded DNA genome of 5.6 kb with long inverted terminal repeats (ITR) at both the 5' and 3' end. Previous attempts to construct a full-length B19 clone were unsuccessful due to deletions in the ITR sequences. We cloned the complete parvovirus B19 genome with intact ITRs from an aplastic crisis patient. Sequence analysis of the complete viral genome indicated that both 5' and 3' ITRs have two sequence configurations and several base changes within the ITRs compared to previous published sequences. After transfection of the plasmid into permissive cells, spliced and non-spliced viral transcripts and viral capsid proteins could be detected. Southern blot analysis of the DNA purified from the plasmid-transfected cells confirmed parvovirus B19 DNA replication. Production of infectious virus by the B19 plasmid was shown by inoculation of cell lysate derived from transfected cells into fresh cells. Together, these results indicate the first successful production of an infectious clone for parvovirus B19 virus. |*Cloning, Molecular[MESH] |3' Untranslated Regions[MESH] |5' Untranslated Regions[MESH] |Base Sequence[MESH] |Cell Line[MESH] |DNA Replication[MESH] |Genome, Viral[MESH] |Humans[MESH] |Molecular Sequence Data[MESH] |Parvovirus B19, Human/*genetics/*pathogenicity[MESH] |Sequence Analysis, DNA[MESH] |Terminal Repeat Sequences/genetics[MESH] |Transcription, Genetic[MESH]Construction and sequencing of an infectious clone of the human parvov(epmc).pdf DeepDyve Pubget Overpricing