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10.1073/pnas.0308434100 http://scihub22266oqcxt.onion/10.1073/pnas.0308434100 14769928!357052!14769928     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Proc+Natl+Acad+Sci+U+S+A 2004 ; 101 (7): 2064-9 Nephropedia Template TP {{tp|p=14769928|t=2004. WNK4 regulates apical and basolateral Cl- flux in extrarenal epithelia |pdf=|usr=}}{{14769928}} gab.com Text WNK4 regulates apical and basolateral Cl- flux in extrarenal epithelia JO: Proc Natl Acad Sci U S A http://www.kidney.de/pget.php?&tw=1&pmid=14769928 #FOAvip Mutations in the serine-threonine kinase WNK4 [with no lysine (K) 4] cause pseudohypoaldosteronism type II, a Mendelian disease featuring hypertension with hyperkalemia. In the kidney, WNK4 regulates the balance between NaCl reabsorption and K(+) secretion via variable inhibition of the thiazide-sensistive NaCl cotransporter and the K(+) channel ROMK. We now demonstrate expression of WNK4 mRNA and protein outside the kidney. In extrarenal tissues, WNK4 is found almost exclusively in polarized epithelia, variably associating with tight junctions, lateral membranes, and cytoplasm. Epithelia expressing WNK4 include sweat ducts, colonic crypts, pancreatic ducts, bile ducts, and epididymis. WNK4 is also expressed in the specialized endothelium of the blood-brain barrier. These epithelia and endothelium all play important roles in Cl(-) transport. Because WNK4 is known to regulate renal Cl(-) handling, we tested WNK4's effect on the activity of mediators of epithelial Cl(-) flux whose extrarenal expression overlaps with WNK4. WNK4 proved to be a potent inhibitor of the activity of both the Na(+)-K(+)-2Cl(-) cotransporter (NKCC1) and the Cl(-)/base exchanger SLC26A6 (CFEX) (>95% inhibition of NKCC1-mediated (86)Rb influx, P < 0.001; >80% inhibition of CFEX-mediated [(14)C] formate uptake, P < 0.001), mediators of Cl(-) flux across basolateral and apical membranes, respectively. In contrast, WNK4 showed no inhibition of pendrin, a related Cl(-)/base exchanger. These findings indicate a general role for WNK4 in the regulation of electrolyte flux in diverse epithelia. Moreover, they reveal that WNK4 regulates the activities of a diverse group of structurally unrelated ion channels, cotransporters, and exchangers. Twit Text FOAVip WNK4 regulates apical and basolateral Cl- flux in extrarenal epithelia ????Proc Natl Acad Sci U S A http://www.kidney.de/pget.php?&tw=1&pmid=14769928 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=14769928 #FOAvip English Wikipedia <ref>{{Cite pmid|14769928}}</ref> WNK4 regulates apical and basolateral Cl- flux in extrarenal epithelia #MMPMID14769928 Kahle KT; Gimenez I; Hassan H; Wilson FH; Wong RD; Forbush B; Aronson PS; Lifton RP Proc Natl Acad Sci U S A 2004[Feb]; 101 (7): 2064-9 PMID14769928show ga Mutations in the serine-threonine kinase WNK4 [with no lysine (K) 4] cause pseudohypoaldosteronism type II, a Mendelian disease featuring hypertension with hyperkalemia. In the kidney, WNK4 regulates the balance between NaCl reabsorption and K(+) secretion via variable inhibition of the thiazide-sensistive NaCl cotransporter and the K(+) channel ROMK. We now demonstrate expression of WNK4 mRNA and protein outside the kidney. In extrarenal tissues, WNK4 is found almost exclusively in polarized epithelia, variably associating with tight junctions, lateral membranes, and cytoplasm. Epithelia expressing WNK4 include sweat ducts, colonic crypts, pancreatic ducts, bile ducts, and epididymis. WNK4 is also expressed in the specialized endothelium of the blood-brain barrier. These epithelia and endothelium all play important roles in Cl(-) transport. Because WNK4 is known to regulate renal Cl(-) handling, we tested WNK4's effect on the activity of mediators of epithelial Cl(-) flux whose extrarenal expression overlaps with WNK4. WNK4 proved to be a potent inhibitor of the activity of both the Na(+)-K(+)-2Cl(-) cotransporter (NKCC1) and the Cl(-)/base exchanger SLC26A6 (CFEX) (>95% inhibition of NKCC1-mediated (86)Rb influx, P < 0.001; >80% inhibition of CFEX-mediated [(14)C] formate uptake, P < 0.001), mediators of Cl(-) flux across basolateral and apical membranes, respectively. In contrast, WNK4 showed no inhibition of pendrin, a related Cl(-)/base exchanger. These findings indicate a general role for WNK4 in the regulation of electrolyte flux in diverse epithelia. Moreover, they reveal that WNK4 regulates the activities of a diverse group of structurally unrelated ion channels, cotransporters, and exchangers. |*Cell Polarity[MESH] |*Membrane Transport Proteins[MESH] |Animals[MESH] |Blotting, Western[MESH] |Carrier Proteins/genetics/metabolism[MESH] |Chlorides/*metabolism[MESH] |Epithelium/*metabolism[MESH] |Humans[MESH] |Immunohistochemistry[MESH] |Ion Transport[MESH] |Kidney/metabolism[MESH] |Membrane Proteins/genetics/metabolism[MESH] |Mice[MESH] |Oocytes/metabolism[MESH] |Protein Serine-Threonine Kinases/genetics/*metabolism[MESH] |RNA, Messenger/genetics/metabolism[MESH] |Sodium-Potassium-Chloride Symporters/genetics/metabolism[MESH] |Solute Carrier Family 12, Member 2[MESH] |Sulfate Transporters[MESH]WNK4 regulates apical and basolateral Cl- flux in extrarenal epithelia(epmc).pdf DeepDyve Pubget Overpricing