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10.4049/jimmunol.172.1.601 http://scihub22266oqcxt.onion/10.4049/jimmunol.172.1.601 14688372!ä!14688372 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Immunol 2004 ; 172 (1): 601-7 Nephropedia Template TP {{tp|p=14688372|t=2004. Cytoskeletal reorganization internalizes multiple transient receptor potential channels and blocks calcium entry into human neutrophils |pdf=|usr=}}{{14688372}} gab.com Text Cytoskeletal reorganization internalizes multiple transient receptor potential channels and blocks calcium entry into human neutrophils JO: J Immunol http://www.kidney.de/pget.php?&tw=1&pmid=14688372 #FOAvip Store-operated calcium entry (SOCE) is required for polymorphonuclear neutrophil (PMN) activation in response to G protein-coupled agonists. Some immunocytes express proteins homologous to the Drosophila transient receptor potential gene (trp) calcium channel. TRP proteins assemble into heterotetrameric ion channels and are known to support SOCE in overexpression systems, but the evidence that TRP proteins support SOCE and are functionally important in wild-type cells remains indirect. We therefore studied the expression and function of TRP proteins in primary human PMN. TRPC1, TRPC3, TRPC4, and TRPC6 were all expressed as mRNA as well as membrane proteins. Immunofluorescence microscopy demonstrated localization of TRPC1, TRPC3, and TRPC4 to the PMN cell membrane and their internalization after cytoskeletal reorganization by calyculin A (CalyA). Either TRPC internalization by CalyA or treatment with the inositol triphosphate receptor inhibitor 2-aminoethoxydiphenyl borane resulted in the loss of PMN SOCE. Cytochalasin D (CytoD) disrupts actin filaments, thus preventing cytoskeletal reorganization, and pretreatment with CytoD rescued PMN SOCE from inhibition by CalyA. Comparative studies of CytoD and 2-aminoethoxydiphenyl borane inhibition of PMN cationic entry after thapsigargin or platelet-activating factor suggested that SOCE occurs through both calcium-specific and nonspecific pathways. Taken together, these studies suggest that the multiple TRPC proteins expressed by human PMN participate in the formation of at least two store-operated calcium channels that have differing ionic permeabilities and regulatory characteristics. Twit Text FOAVip Cytoskeletal reorganization internalizes multiple transient receptor potential channels and blocks calcium entry into human neutrophils ????J Immunol http://www.kidney.de/pget.php?&tw=1&pmid=14688372 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=14688372 #FOAvip English Wikipedia <ref>{{Cite pmid|14688372}}</ref> Cytoskeletal reorganization internalizes multiple transient receptor potential channels and blocks calcium entry into human neutrophils #MMPMID14688372 Itagaki K; Kannan KB; Singh BB; Hauser CJ J Immunol 2004[Jan]; 172 (1): 601-7 PMID14688372show ga Store-operated calcium entry (SOCE) is required for polymorphonuclear neutrophil (PMN) activation in response to G protein-coupled agonists. Some immunocytes express proteins homologous to the Drosophila transient receptor potential gene (trp) calcium channel. TRP proteins assemble into heterotetrameric ion channels and are known to support SOCE in overexpression systems, but the evidence that TRP proteins support SOCE and are functionally important in wild-type cells remains indirect. We therefore studied the expression and function of TRP proteins in primary human PMN. TRPC1, TRPC3, TRPC4, and TRPC6 were all expressed as mRNA as well as membrane proteins. Immunofluorescence microscopy demonstrated localization of TRPC1, TRPC3, and TRPC4 to the PMN cell membrane and their internalization after cytoskeletal reorganization by calyculin A (CalyA). Either TRPC internalization by CalyA or treatment with the inositol triphosphate receptor inhibitor 2-aminoethoxydiphenyl borane resulted in the loss of PMN SOCE. Cytochalasin D (CytoD) disrupts actin filaments, thus preventing cytoskeletal reorganization, and pretreatment with CytoD rescued PMN SOCE from inhibition by CalyA. Comparative studies of CytoD and 2-aminoethoxydiphenyl borane inhibition of PMN cationic entry after thapsigargin or platelet-activating factor suggested that SOCE occurs through both calcium-specific and nonspecific pathways. Taken together, these studies suggest that the multiple TRPC proteins expressed by human PMN participate in the formation of at least two store-operated calcium channels that have differing ionic permeabilities and regulatory characteristics. |Calcium Channel Blockers/*metabolism/pharmacology[MESH] |Calcium Channels/biosynthesis/genetics/*metabolism[MESH] |Calcium Signaling/drug effects/immunology[MESH] |Calcium/*antagonists & inhibitors/*metabolism[MESH] |Cation Transport Proteins/biosynthesis/genetics/metabolism[MESH] |Cytoskeleton/drug effects/*metabolism[MESH] |Endocytosis/drug effects/*immunology[MESH] |Gene Expression Regulation/drug effects/immunology[MESH] |Humans[MESH] |Immunoblotting[MESH] |Inositol 1,4,5-Trisphosphate Receptors[MESH] |Ion Channels/biosynthesis/genetics/metabolism[MESH] |Marine Toxins[MESH] |Neutrophil Activation/immunology[MESH] |Neutrophils/drug effects/*metabolism[MESH] |Oxazoles/pharmacology[MESH] |Protein Isoforms/biosynthesis/genetics/metabolism[MESH] |Receptors, Cytoplasmic and Nuclear/metabolism[MESH] |Receptors, G-Protein-Coupled/agonists[MESH] |TRPC Cation Channels[MESH]Cytoskeletal reorganization internalizes multiple transient receptor p(epmc).pdf DeepDyve Pubget Overpricing