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10.1016/j.bbrc.2003.11.064 http://scihub22266oqcxt.onion/10.1016/j.bbrc.2003.11.064 14652014!7111096!14652014     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Biochem+Biophys+Res+Commun 2003 ; 312 (4): 1290-6 Nephropedia Template TP {{tp|p=14652014|t=2003. A real-time PCR for SARS-coronavirus incorporating target gene pre-amplification |pdf=|usr=}}{{14652014}} gab.com Text A real-time PCR for SARS-coronavirus incorporating target gene pre-amplification JO: Biochem Biophys Res Commun http://www.kidney.de/pget.php?&tw=1&pmid=14652014 #FOAvip An enhanced polymerase chain reaction (PCR) assay to detect the coronavirus associated with severe acute respiratory syndrome (SARS-CoV) was developed in which a target gene pre-amplification step preceded TaqMan real-time fluorescent PCR. Clinical samples were collected from 120 patients diagnosed as suspected or probable SARS cases and analyzed by conventional PCR followed by agarose gel electrophoresis, conventional TaqMan real-time PCR, and our enhanced TaqMan real-time PCR assays. An amplicon of the size expected from SARS-CoV was obtained from 28/120 samples using the enhanced real-time PCR method. Conventional PCR and real-time PCR alone identified fewer SARS-CoV positive cases. Results were confirmed by viral culture in 3/28 cases. The limit of detection of the enhanced real-time PCR method was 10(2)-fold higher than the standard real-time PCR assay and 10(7)-fold higher than conventional PCR methods. The increased sensitivity of the assay may help control the spread of the disease during future SARS outbreaks. Twit Text FOAVip A real-time PCR for SARS-coronavirus incorporating target gene pre-amplification ????Biochem Biophys Res Commun http://www.kidney.de/pget.php?&tw=1&pmid=14652014 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=14652014 #FOAvip English Wikipedia <ref>{{Cite pmid|14652014}}</ref> A real-time PCR for SARS-coronavirus incorporating target gene pre-amplification #MMPMID14652014 Lau LT; Fung YW; Wong FP; Lin SS; Wang CR; Li HL; Dillon N; Collins RA; Tam JS; Chan PK; Wang CG; Yu AC Biochem Biophys Res Commun 2003[Dec]; 312 (4): 1290-6 PMID14652014show ga An enhanced polymerase chain reaction (PCR) assay to detect the coronavirus associated with severe acute respiratory syndrome (SARS-CoV) was developed in which a target gene pre-amplification step preceded TaqMan real-time fluorescent PCR. Clinical samples were collected from 120 patients diagnosed as suspected or probable SARS cases and analyzed by conventional PCR followed by agarose gel electrophoresis, conventional TaqMan real-time PCR, and our enhanced TaqMan real-time PCR assays. An amplicon of the size expected from SARS-CoV was obtained from 28/120 samples using the enhanced real-time PCR method. Conventional PCR and real-time PCR alone identified fewer SARS-CoV positive cases. Results were confirmed by viral culture in 3/28 cases. The limit of detection of the enhanced real-time PCR method was 10(2)-fold higher than the standard real-time PCR assay and 10(7)-fold higher than conventional PCR methods. The increased sensitivity of the assay may help control the spread of the disease during future SARS outbreaks. |Genetic Testing/*methods[MESH] |Genome, Viral[MESH] |Humans[MESH] |Nucleic Acid Amplification Techniques/methods[MESH] |Online Systems[MESH] |Polymerase Chain Reaction/*methods[MESH] |Reproducibility of Results[MESH] |Sensitivity and Specificity[MESH] |Severe Acute Respiratory Syndrome/*diagnosis/virology[MESH]A real-time PCR for SARS-coronavirus incorporating target gene pre-amp(epmc).pdf DeepDyve Pubget Overpricing