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10.1002/jemt.1070210109 http://scihub22266oqcxt.onion/10.1002/jemt.1070210109 1375519!ä!1375519 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Microsc+Res+Tech 1992 ; 21 (1): 59-64 Nephropedia Template TP {{tp|p=1375519|t=1992. A combined pseudoreplica-immunochemical technique for research and diagnostic virology |pdf=|usr=}}{{1375519}} gab.com Text A combined pseudoreplica-immunochemical technique for research and diagnostic virology JO: Microsc Res Tech http://www.kidney.de/pget.php?&tw=1&pmid=1375519 #FOAvip Pseudoreplica and immunochemical techniques were combined in a single protocol for identification of virus in research and clinical specimens. Stock preparations of vesicular stomatitis virus (VSV), cytomegalovirus (CMV), and herpes simplex virus (HSV) were used to develop the technique. Traditional pseudoreplicas of viral stock solutions were prepared but not negatively stained. The virus was then immunolabeled in two stages. Virus-specific polyclonal antisera were used in the first stage; colloidal gold conjugated antibodies were used as indicator antibody in the second stage. After immunolabeling, the grids were negatively stained. As a demonstration of the clinical usefulness of this approach, it was employed to antenatally identify human parvovirus B19 particles in ascites from a 22 week gestational fetus with nonimmune hydrops fetalis. The combined pseudoreplica-immunochemical approach offers several advantages over both the pseudoreplica and immunochemical methods when used in isolation. Advantages include relative purification of samples, concentration of virus, morphological preservation, and enhanced diagnostic specificity. Twit Text FOAVip A combined pseudoreplica-immunochemical technique for research and diagnostic virology ????Microsc Res Tech http://www.kidney.de/pget.php?&tw=1&pmid=1375519 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=1375519 #FOAvip English Wikipedia <ref>{{Cite pmid|1375519}}</ref> A combined pseudoreplica-immunochemical technique for research and diagnostic virology #MMPMID1375519 Fritz B; Moore K; Naides SJ Microsc Res Tech 1992[Mar]; 21 (1): 59-64 PMID1375519show ga Pseudoreplica and immunochemical techniques were combined in a single protocol for identification of virus in research and clinical specimens. Stock preparations of vesicular stomatitis virus (VSV), cytomegalovirus (CMV), and herpes simplex virus (HSV) were used to develop the technique. Traditional pseudoreplicas of viral stock solutions were prepared but not negatively stained. The virus was then immunolabeled in two stages. Virus-specific polyclonal antisera were used in the first stage; colloidal gold conjugated antibodies were used as indicator antibody in the second stage. After immunolabeling, the grids were negatively stained. As a demonstration of the clinical usefulness of this approach, it was employed to antenatally identify human parvovirus B19 particles in ascites from a 22 week gestational fetus with nonimmune hydrops fetalis. The combined pseudoreplica-immunochemical approach offers several advantages over both the pseudoreplica and immunochemical methods when used in isolation. Advantages include relative purification of samples, concentration of virus, morphological preservation, and enhanced diagnostic specificity. |*Replica Techniques[MESH] |Cytomegalovirus/isolation & purification[MESH] |Immunochemistry[MESH] |Negative Staining[MESH] |Parvovirus B19, Human/isolation & purification[MESH] |Research[MESH] |Simplexvirus/isolation & purification[MESH] |Staining and Labeling[MESH] |Vesicular stomatitis Indiana virus/isolation & purification[MESH]A combined pseudoreplica-immunochemical technique for research and dia(epmc).pdf DeepDyve Pubget Overpricing