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10.1084/jem.113.2.405 http://scihub22266oqcxt.onion/10.1084/jem.113.2.405 13692336!2137358!13692336     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=13692336&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215       J+Exp+Med 1961 ; 113 (2): 405-17 Nephropedia Template TP {{tp|p=13692336|t=1961. A comparative study of the growth, nutrition, and metabolism of the primary and the transformed human cells in vitro |pdf=|usr=}}{{13692336}} gab.com Text A comparative study of the growth, nutrition, and metabolism of the primary and the transformed human cells in vitro JO: J Exp Med http://www.kidney.de/pget.php?&tw=1&pmid=13692336 #FOAvip The growth characteristics, nutritional requirements, and metabolic activities of primary explants (chiefly amnion) and transformed human cells (derived from amnion, conjunctiva, and cervical cancer) were compared. Major differences observed are as follows: 1. Five types of human tissue when freshly explanted in vitro were found to have varying but limited growth potential in contrast to the autonomous and apparently unlimited growth of the transformed cell. 2. A requirement for an external source of inositol or glutamine could not be demonstrated for the slowly multiplying primary amnions in contrast to the rapidly growing transformed cells which degenerated in the absence of either metabolite. 3. Unlike the transformed cells, the primary amnion cells fixed an insignificant amount of CO(2); when infected by the Coxsackie virus, however, a requirement for CO(2) in the formation of more virus became readily demonstrable. 4. The biggest difference in the incorporation of various C(14) substrates occurred in the nucleic acid fractions. 5. The difference in the incorporation of glycine into the protein fraction was very small. 6. The utilization of glucose, ribose, and xylose as well as the oxidation of lactic acid into CO(2) by the primary amnion cells occurred at a rate much lower than that of the transformed cells. 7. The cytotoxic dose of 5-fluorodeoxyuridine for the primary amnion was at least 100 times that of the transformed cells although growth inhibition was readily demonstrated at 0.1 microg. per ml. for both types of cells. Based on these observations, several hypotheses are put forth for consideration. Twit Text FOAVip A comparative study of the growth, nutrition, and metabolism of the primary and the transformed human cells in vitro ????J Exp Med http://www.kidney.de/pget.php?&tw=1&pmid=13692336 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=13692336 #FOAvip English Wikipedia <ref>{{Cite pmid|13692336}}</ref> A comparative study of the growth, nutrition, and metabolism of the primary and the transformed human cells in vitro #MMPMID13692336 CHANG RS J Exp Med 1961[Feb]; 113 (2): 405-17 PMID13692336show ga The growth characteristics, nutritional requirements, and metabolic activities of primary explants (chiefly amnion) and transformed human cells (derived from amnion, conjunctiva, and cervical cancer) were compared. Major differences observed are as follows: 1. Five types of human tissue when freshly explanted in vitro were found to have varying but limited growth potential in contrast to the autonomous and apparently unlimited growth of the transformed cell. 2. A requirement for an external source of inositol or glutamine could not be demonstrated for the slowly multiplying primary amnions in contrast to the rapidly growing transformed cells which degenerated in the absence of either metabolite. 3. Unlike the transformed cells, the primary amnion cells fixed an insignificant amount of CO(2); when infected by the Coxsackie virus, however, a requirement for CO(2) in the formation of more virus became readily demonstrable. 4. The biggest difference in the incorporation of various C(14) substrates occurred in the nucleic acid fractions. 5. The difference in the incorporation of glycine into the protein fraction was very small. 6. The utilization of glucose, ribose, and xylose as well as the oxidation of lactic acid into CO(2) by the primary amnion cells occurred at a rate much lower than that of the transformed cells. 7. The cytotoxic dose of 5-fluorodeoxyuridine for the primary amnion was at least 100 times that of the transformed cells although growth inhibition was readily demonstrated at 0.1 microg. per ml. for both types of cells. Based on these observations, several hypotheses are put forth for consideration. |*Amnion[MESH] |*Glucose[MESH] |*Oxidation-Reduction[MESH] |*Proteins[MESH] |*Tissue Culture Techniques[MESH] |Humans[MESH]A comparative study of the growth, nutrition, and metabolism of the pr(epmc).pdf DeepDyve Pubget Overpricing