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pmid12635865      Magnes+Res 2002 ; 15 (3-4): 147-52
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  • Effects of a low extracellular magnesium concentration and endotoxin on IL-1beta and TNF-alpha release from, and mRNA levels in, isolated rat alveolar macrophages #MMPMID12635865
  • Shogi T; Oono H; Nakagawa M; Miyamoto A; Ishiguro S; Nishio A
  • Magnes Res 2002[Dec]; 15 (3-4): 147-52 PMID12635865show ga
  • The aim of this study was to assess the effects of a low extracellular Mg2+ concentration and endotoxin on interleukin (IL)-1beta and tumour necrosis factor (TNF)-alpha release from, and mRNA levels in, rat alveolar macrophages. A low extracellular Mg2+ concentration enhanced the release of both cytokines, and this release was suppressed by a calcium antagonist, nifedipine. Bacterial lipopolysaccharide (LPS) also enhanced the release of both cytokines, and this enhancement was stronger in a low-Mg2+ medium than in control medium. Furthermore, LPS increased the mRNA levels of both cytokines in alveolar macrophages, and this increase was enhanced in low-Mg2+ medium. These results suggest that a low extracellular Mg2+ concentration and LPS stimulate the release of IL-1beta and TNF-alpha from rat alveolar macrophages by increasing the synthesis of both cytokines and by Ca2+ signaling pathways.
  • |Animals[MESH]
  • |Culture Media[MESH]
  • |DNA Primers[MESH]
  • |Endotoxins/*pharmacology[MESH]
  • |In Vitro Techniques[MESH]
  • |Interleukin-1/*biosynthesis[MESH]
  • |Macrophages, Alveolar/cytology/drug effects/*metabolism[MESH]
  • |Magnesium/metabolism/*pharmacology[MESH]
  • |Male[MESH]
  • |RNA, Messenger/*biosynthesis[MESH]
  • |Rats[MESH]
  • |Rats, Wistar[MESH]


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