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10.1016/s0024-3205(02)02371-8 http://scihub22266oqcxt.onion/10.1016/s0024-3205(02)02371-8 12570925!ä!12570925 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Life+Sci 2003 ; 72 (11): 1247-57 Nephropedia Template TP {{tp|p=12570925|t=2003. Magnesium-deficient medium enhances NO production in alveolar macrophages isolated from rats |pdf=|usr=}}{{12570925}} gab.com Text Magnesium-deficient medium enhances NO production in alveolar macrophages isolated from rats JO: Life Sci http://www.kidney.de/pget.php?&tw=1&pmid=12570925 #FOAvip Magnesium deficiency has been shown to increase nitric oxide (NO) levels in plasma and to aggravate endotoxin lethality. The present study was performed to examine the effects of magnesium (Mg(2+))-deficient culture medium, with and without endotoxin (LPS), on NO release and inducible NOS (iNOS) mRNA levels in alveolar macrophages isolated from rats. Decreasing the Mg(2+) concentration in the culture medium from 0.39 mM (normal-Mg(2+) medium) to 0.021 mM (Mg(2+)-deficient medium) increased NO release from alveolar macrophages for 2 h. However, LPS stimulation in Mg(2+)-deficient medium had little effect on NO release. The increased NO release in Mg(2+)-deficient medium was suppressed completely by L-NAME and aminoguanidine. Dexamethasone, pyrrolidine dithiocarbamate and curcumin strongly inhibited NO release. Verapamil, U73122, TMB-8 and W-7 had no significant effect on NO release induced by Mg(2+) deficiency. Preculture of macrophages with Mg(2+)-deficient medium for 22 h markedly increased NO release and iNOS mRNA levels for a further 2 h; these increments were suppressed completely by curcumin. These results suggest that Mg(2+) deficiency enhances NO production via iNOS by alveolar macrophages. In this experimental condition, we can not suggest that NO production from alveolar macrophage plays an essential role in the pathogenesis of enhanced endotoxin lethality in Mg-deficient rats. Twit Text FOAVip Magnesium-deficient medium enhances NO production in alveolar macrophages isolated from rats ????Life Sci http://www.kidney.de/pget.php?&tw=1&pmid=12570925 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=12570925 #FOAvip English Wikipedia <ref>{{Cite pmid|12570925}}</ref> Magnesium-deficient medium enhances NO production in alveolar macrophages isolated from rats #MMPMID12570925 Yokoyama T; Oono H; Miyamoto A; Ishiguro S; Nishio A Life Sci 2003[Jan]; 72 (11): 1247-57 PMID12570925show ga Magnesium deficiency has been shown to increase nitric oxide (NO) levels in plasma and to aggravate endotoxin lethality. The present study was performed to examine the effects of magnesium (Mg(2+))-deficient culture medium, with and without endotoxin (LPS), on NO release and inducible NOS (iNOS) mRNA levels in alveolar macrophages isolated from rats. Decreasing the Mg(2+) concentration in the culture medium from 0.39 mM (normal-Mg(2+) medium) to 0.021 mM (Mg(2+)-deficient medium) increased NO release from alveolar macrophages for 2 h. However, LPS stimulation in Mg(2+)-deficient medium had little effect on NO release. The increased NO release in Mg(2+)-deficient medium was suppressed completely by L-NAME and aminoguanidine. Dexamethasone, pyrrolidine dithiocarbamate and curcumin strongly inhibited NO release. Verapamil, U73122, TMB-8 and W-7 had no significant effect on NO release induced by Mg(2+) deficiency. Preculture of macrophages with Mg(2+)-deficient medium for 22 h markedly increased NO release and iNOS mRNA levels for a further 2 h; these increments were suppressed completely by curcumin. These results suggest that Mg(2+) deficiency enhances NO production via iNOS by alveolar macrophages. In this experimental condition, we can not suggest that NO production from alveolar macrophage plays an essential role in the pathogenesis of enhanced endotoxin lethality in Mg-deficient rats. |Animals[MESH] |Calcium Signaling[MESH] |Cells, Cultured[MESH] |Culture Media[MESH] |Lipopolysaccharides/pharmacology[MESH] |Macrophages, Alveolar/*metabolism[MESH] |Magnesium Deficiency/*metabolism[MESH] |Male[MESH] |NG-Nitroarginine Methyl Ester/pharmacology[MESH] |Nitric Oxide Synthase Type II[MESH] |Nitric Oxide Synthase/genetics[MESH] |Nitric Oxide/*biosynthesis[MESH]Magnesium-deficient medium enhances NO production in alveolar macropha(epmc).pdf DeepDyve Pubget Overpricing