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10.1016/s0166-0934(02)00195-7 http://scihub22266oqcxt.onion/10.1016/s0166-0934(02)00195-7 12445941!ä!12445941 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Virol+Methods 2003 ; 107 (1): 81-7 Nephropedia Template TP {{tp|p=12445941|t=2003. Evaluation of anti-parvovirus B19 activity in sera by assay using quantitative polymerase chain reaction |pdf=|usr=}}{{12445941}} gab.com Text Evaluation of anti-parvovirus B19 activity in sera by assay using quantitative polymerase chain reaction JO: J Virol Methods http://www.kidney.de/pget.php?&tw=1&pmid=12445941 #FOAvip Human parvovirus B19 (B19) infects cells of erythroid lineage. Production of neutralizing antibodies (Abs) is indispensable for recovery from B19-related disease state. In this study, we used a convenient method to measure neutralizing activities in human sera by using a real-time quantitative PCR based assay. Erythroid cell line KU812Ep6 was incubated with test sera before infection with B19 virus. The copy number of B19-DNA in cultures was decreased in the presence of the sera from patients who recovered from acute B19 infection, whereas no decrease in B19-DNA was in cultures incubated with sera from healthy volunteers who had no B19 infection. The decrease in B19-DNA copy number was calculated and the inhibition percentage was expressed as neutralizing activity to B19. A clinical study showed that the levels of neutralizing ability were high in patients who recovered soon after acute B19 infection, but were low in some patients with a prolonged clinical course for recovery from B19 infection. This method is simple and convenient compared with methods described previously, showing its usefulness to evaluate the neutralizing activity to B19. Twit Text FOAVip Evaluation of anti-parvovirus B19 activity in sera by assay using quantitative polymerase chain reaction ????J Virol Methods http://www.kidney.de/pget.php?&tw=1&pmid=12445941 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=12445941 #FOAvip English Wikipedia <ref>{{Cite pmid|12445941}}</ref> Evaluation of anti-parvovirus B19 activity in sera by assay using quantitative polymerase chain reaction #MMPMID12445941 Saito T; Munakata Y; Fu Y; Fujii H; Kodera T; Miyagawa E; Ishii K; Sasaki T J Virol Methods 2003[Jan]; 107 (1): 81-7 PMID12445941show ga Human parvovirus B19 (B19) infects cells of erythroid lineage. Production of neutralizing antibodies (Abs) is indispensable for recovery from B19-related disease state. In this study, we used a convenient method to measure neutralizing activities in human sera by using a real-time quantitative PCR based assay. Erythroid cell line KU812Ep6 was incubated with test sera before infection with B19 virus. The copy number of B19-DNA in cultures was decreased in the presence of the sera from patients who recovered from acute B19 infection, whereas no decrease in B19-DNA was in cultures incubated with sera from healthy volunteers who had no B19 infection. The decrease in B19-DNA copy number was calculated and the inhibition percentage was expressed as neutralizing activity to B19. A clinical study showed that the levels of neutralizing ability were high in patients who recovered soon after acute B19 infection, but were low in some patients with a prolonged clinical course for recovery from B19 infection. This method is simple and convenient compared with methods described previously, showing its usefulness to evaluate the neutralizing activity to B19. |Animals[MESH] |Antibodies, Viral/*blood[MESH] |Humans[MESH] |Mice[MESH] |Mice, Inbred BALB C[MESH] |Neutralization Tests[MESH] |Parvovirus B19, Human/*immunology[MESH]Evaluation of anti-parvovirus B19 activity in sera by assay using quan(epmc).pdf DeepDyve Pubget Overpricing