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10.1086/344355

http://scihub22266oqcxt.onion/10.1086/344355
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12404168!ä!12404168

suck abstract from ncbi


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pmid12404168      J+Infect+Dis 2002 ; 186 (10): 1494-7
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  • Human parvovirus Bb19 detection in asymptomatic blood donors: association with increased neopterin concentrations #MMPMID12404168
  • Schennach H; Lanthaler AJ; Mayersbach P; Ulmer H; Muell K; Schoenitzer D; Fuchs D; Larcher C
  • J Infect Dis 2002[Nov]; 186 (10): 1494-7 PMID12404168show ga
  • Serum neopterin concentrations were determined in 20,000 blood donations. For the 400 donations with neopterin concentrations above the 98 th percentile and another 1200 donations with neopterin concentrations in the lower range, results of human parvovirus (HPV) B19 tests were compared. Infectious specimens were identified by dot blot hybridization assay and polymerase chain reaction (PCR) that used the outer primers and detected 1 pg of HPV B19 DNA, corresponding to approximately 10(5) copies of the genome, in the specimens and by a nested PCR that detected 1-10 fg of DNA, corresponding to 10(2)-10(3) copies of the genome. Of 400 specimens with neopterin concentrations > or =12 nmol/L (98th percentile, current cutoff), 10 tested positive by dot blot hybridization assay (9 of these were confirmed by nested PCR). Among 1200 specimens with low neopterin concentrations (<12 nmol/L), no specimen containing HPV B19 DNA was detected. These findings suggest an association between elevated neopterin concentrations and HPV B19 infectivity.
  • |*Blood Donors[MESH]
  • |Enzyme-Linked Immunosorbent Assay[MESH]
  • |Humans[MESH]
  • |Neopterin/*metabolism[MESH]
  • |Parvoviridae Infections/immunology/*metabolism/virology[MESH]
  • |Parvovirus B19, Human/genetics/immunology/*isolation & purification[MESH]


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