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10.1016/s0006-3223(02)01387-2 http://scihub22266oqcxt.onion/10.1016/s0006-3223(02)01387-2 12062882!ä!12062882 Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=12062882&cmd=llinks): Failed to open stream: HTTP request failed! 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High-avidity human serum antibodies recognizing linear epitopes of Borna disease virus proteins |pdf=|usr=}}{{12062882}} gab.com Text High-avidity human serum antibodies recognizing linear epitopes of Borna disease virus proteins JO: Biol Psychiatry http://www.kidney.de/pget.php?&tw=1&pmid=12062882 #FOAvip BACKGROUND: The recent observation that Borna disease virus (BDV)-reactive antibodies from psychiatric patients exhibit only low avidity for BDV antigen called into question their diagnostic value and raised the possibility that antigenically related microorganisms or self antigens caused the production of these antibodies. We further characterized the specificity of these antibodies. METHODS: We established a peptide array-based screening test that allows the identification of antibodies directed against linear epitopes of the two major BDV proteins, the nucleoprotein (N) and the phosphoprotein (P). RESULTS: Initial tests employing sera of BDV-infected mice and rats or horses with Borna disease revealed a high specificity and sensitivity of this test. All sera recognized epitopes of N, P, or both. Sera of noninfected rats, mice, and horses showed no signals on either peptide array. Several human sera that recognized BDV antigen by indirect immunofluorescence contained antibodies that recognized various linear epitopes of one or even both BDV proteins. Remarkably, antibodies purified from such human serum by matrix-immobilized peptides showed high-avidity binding to BDV antigens when assayed by IFA or Western blotting. CONCLUSIONS: These data suggest that reactive antibodies found in psychiatric patients indeed indicate infection with BDV or a BDV-like agent. However, the poor affinity maturation of BDV-specific human antibodies remains unexplained. Twit Text FOAVip High-avidity human serum antibodies recognizing linear epitopes of Borna disease virus proteins ????Biol Psychiatry http://www.kidney.de/pget.php?&tw=1&pmid=12062882 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=12062882 #FOAvip English Wikipedia <ref>{{Cite pmid|12062882}}</ref> High-avidity human serum antibodies recognizing linear epitopes of Borna disease virus proteins #MMPMID12062882 Billich C; Sauder C; Frank R; Herzog S; Bechter K; Takahashi K; Peters H; Staeheli P; Schwemmle M Biol Psychiatry 2002[Jun]; 51 (12): 979-87 PMID12062882show ga BACKGROUND: The recent observation that Borna disease virus (BDV)-reactive antibodies from psychiatric patients exhibit only low avidity for BDV antigen called into question their diagnostic value and raised the possibility that antigenically related microorganisms or self antigens caused the production of these antibodies. We further characterized the specificity of these antibodies. METHODS: We established a peptide array-based screening test that allows the identification of antibodies directed against linear epitopes of the two major BDV proteins, the nucleoprotein (N) and the phosphoprotein (P). RESULTS: Initial tests employing sera of BDV-infected mice and rats or horses with Borna disease revealed a high specificity and sensitivity of this test. All sera recognized epitopes of N, P, or both. Sera of noninfected rats, mice, and horses showed no signals on either peptide array. Several human sera that recognized BDV antigen by indirect immunofluorescence contained antibodies that recognized various linear epitopes of one or even both BDV proteins. Remarkably, antibodies purified from such human serum by matrix-immobilized peptides showed high-avidity binding to BDV antigens when assayed by IFA or Western blotting. CONCLUSIONS: These data suggest that reactive antibodies found in psychiatric patients indeed indicate infection with BDV or a BDV-like agent. However, the poor affinity maturation of BDV-specific human antibodies remains unexplained. |Animals[MESH] |Antibodies, Viral/*blood[MESH] |Antibody Affinity[MESH] |Antigens, Viral/*immunology[MESH] |Borna Disease/blood/complications/*diagnosis/immunology[MESH] |Borna disease virus/*immunology[MESH] |Epitopes[MESH] |Female[MESH] |Fluorescent Antibody Technique[MESH] |Horses[MESH] |Humans[MESH] |Male[MESH] |Mental Disorders/*virology[MESH] |Mice[MESH] |Molecular Probe Techniques[MESH] |Rats[MESH]High-avidity human serum antibodies recognizing linear epitopes of Bor(epmc).pdf DeepDyve Pubget Overpricing