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10.1099/0022-1317-51-6-484 http://scihub22266oqcxt.onion/10.1099/0022-1317-51-6-484 12018655!ä!12018655 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Med+Microbiol 2002 ; 51 (6): 484-490 Nephropedia Template TP {{tp|p=12018655|t=2002. Role of the respiratory burst in co-operative reduction in neutrophil survival by influenza A virus and Escherichia coli |pdf=|usr=}}{{12018655}} gab.com Text Role of the respiratory burst in co-operative reduction in neutrophil survival by influenza A virus and Escherichia coli JO: J Med Microbiol http://www.kidney.de/pget.php?&tw=1&pmid=12018655 #FOAvip Influenza A virus (IAV)-induced impairment of neutrophil function or survival may be a cause of bacterial superinfection of IAV-infected subjects. This study was performed to determine the mechanism through which the combination of IAV and Escherichia coli co-operatively reduces neutrophil survival. Neutrophil binding of annexin-V and caspase-3 activation was significantly increased by either IAV or E. coli, supporting the concept that the micro-organisms accelerate neutrophil apoptosis. The anti-apoptotic agent granulocyte-macrophage colony stimulating factor (GM-CSF) did not improve, but further reduced, survival of neutrophils treated with IAV and E. coli. As addition of E. coli resulted in greater neutrophil uptake of IAV and greater neutrophil respiratory burst responses to IAV, this study tested whether respiratory burst activation by IAV and E. coli contributes to reducing neutrophil survival. The cell-permeant NADPH oxidase inhibitor, diphenylene iodonium, significantly increased survival of neutrophils treated with either E. coli alone or the combination of IAV and E. coli. In contrast, catalase, which is not cell permeant, did not alter survival of E. coli- and IAV-treated neutrophils. Azide enhanced neutrophil hydrogen peroxide responses to IAV and E. coli, and reduced survival of these cells. These results indicate that co-operative induction of intracellular respiratory burst responses by IAV and E.coli mediates the reduced neutrophil survival caused by these pathogens in vitro. Twit Text FOAVip Role of the respiratory burst in co-operative reduction in neutrophil survival by influenza A virus and Escherichia coli ????J Med Microbiol http://www.kidney.de/pget.php?&tw=1&pmid=12018655 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=12018655 #FOAvip English Wikipedia <ref>{{Cite pmid|12018655}}</ref> Role of the respiratory burst in co-operative reduction in neutrophil survival by influenza A virus and Escherichia coli #MMPMID12018655 Engelich G; White M; Hartshorn KL J Med Microbiol 2002[Jun]; 51 (6): 484-490 PMID12018655show ga Influenza A virus (IAV)-induced impairment of neutrophil function or survival may be a cause of bacterial superinfection of IAV-infected subjects. This study was performed to determine the mechanism through which the combination of IAV and Escherichia coli co-operatively reduces neutrophil survival. Neutrophil binding of annexin-V and caspase-3 activation was significantly increased by either IAV or E. coli, supporting the concept that the micro-organisms accelerate neutrophil apoptosis. The anti-apoptotic agent granulocyte-macrophage colony stimulating factor (GM-CSF) did not improve, but further reduced, survival of neutrophils treated with IAV and E. coli. As addition of E. coli resulted in greater neutrophil uptake of IAV and greater neutrophil respiratory burst responses to IAV, this study tested whether respiratory burst activation by IAV and E. coli contributes to reducing neutrophil survival. The cell-permeant NADPH oxidase inhibitor, diphenylene iodonium, significantly increased survival of neutrophils treated with either E. coli alone or the combination of IAV and E. coli. In contrast, catalase, which is not cell permeant, did not alter survival of E. coli- and IAV-treated neutrophils. Azide enhanced neutrophil hydrogen peroxide responses to IAV and E. coli, and reduced survival of these cells. These results indicate that co-operative induction of intracellular respiratory burst responses by IAV and E.coli mediates the reduced neutrophil survival caused by these pathogens in vitro. |Annexin A5/metabolism[MESH] |Apoptosis[MESH] |Caspase 3[MESH] |Caspases/metabolism[MESH] |Cells, Cultured[MESH] |Enzyme Inhibitors/metabolism[MESH] |Escherichia coli Infections/*complications/immunology[MESH] |Escherichia coli/*immunology[MESH] |Granulocyte-Macrophage Colony-Stimulating Factor/physiology[MESH] |Humans[MESH] |Influenza A virus/*immunology[MESH] |Influenza, Human/*complications/immunology[MESH] |Neutrophils/microbiology/*physiology/virology[MESH] |Respiratory Burst/*physiology[MESH] |Superinfection/etiology[MESH]Role of the respiratory burst in co-operative reduction in neutrophil (epmc).pdf DeepDyve Pubget Overpricing